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KLF9 Recombinant antibody

KLF9 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230436H2

Cat no : 83183-4-RR

Print datasheet

Synonyms

GC-box-binding protein 1, BTE-binding protein 1, BTEB1, BTEB, Basic transcription element-binding protein 1

验证数据展示

  • WB analysis using 83183-4-RR

    WB analysis using 83183-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83183-4-RR (KLF9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of A549 using 83183-4-RR

    FC experiment of A549 using 83183-4-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug KLF9 Recombinant antibody (83183-4-RR, Clone:230436H2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83183-4-RR

    Affinity and Kinetic Characterization of 83183-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83183-4-RR against Human KLF9 were performed. The affinity constant is 0.767 nM.

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, HepG2 cells, A549 cells, U2OS cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83183-4-RR targets KLF9 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen KLF9 fusion protein Ag34411 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Kruppel-like factor 9
Synonyms GC-box-binding protein 1, BTE-binding protein 1, BTEB1, BTEB, Basic transcription element-binding protein 1
Calculated Molecular Weight27 kDa
Observed Molecular Weight27-35 kDa
GenBank Accession NumberBC074880
Gene Symbol KLF9
Gene ID (NCBI) 687
RRIDAB_3670873
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13886
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Krüppel-like factor 9 (KLF9) was a member of SP/KLF family of DNA-binding transcriptional regulators featured by a highly homologous C-terminal three C2H2-type zinc finger DNA-binding domains. KLF9 has been previously reported to be downregulated in various types of human malignancy and serves as a tumour suppressor at tumour onset and development, affecting cell proliferation, apoptosis, metastasis and tumorigenicity (PMID: 32855660).

实验方案

Product Specific Protocols
WB protocol for KLF9 antibody 83183-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols