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KLF9 Recombinant antibody, PBS Only (Detector)

KLF9 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

230436H2

Cat no : 83183-4-PBS

Print datasheet

Synonyms

GC-box-binding protein 1, BTE-binding protein 1, BTEB1, BTEB, Basic transcription element-binding protein 1

验证数据展示

  • WB analysis using 83183-4-RR (same clone as 83183-4-PBS)

    WB analysis using 83183-4-RR (same clone as 83183-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83183-4-RR (KLF9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83183-4-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83183-4-RR (same clone as 83183-4-PBS)

    FC experiment of A549 using 83183-4-RR (same clone as 83183-4-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug KLF9 Recombinant antibody (83183-4-RR, Clone:230436H2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83183-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00148-1

    Cytometric bead array standard curve of MP00148-1

    Cytometric bead array standard curve ofMP00148-1, KLF9 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83183-3-PBS. Detection antibody: 83183-4-PBS. Standard: Ag34411. Range: 0.625-20 ng/mL.

  • Cytometric bead array standard curve of MP00148-2

    Cytometric bead array standard curve of MP00148-2

    Cytometric bead array standard curve ofMP00148-2, KLF9 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83183-2-PBS. Detection antibody: 83183-4-PBS. Standard: Ag34411. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83183-4-RR

    Affinity and Kinetic Characterization of 83183-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83183-4-RR against Human KLF9 were performed. The affinity constant is 0.767 nM.

产品信息

83183-4-PBS targets KLF9 as part of a matched antibody pair:

MP00148-1: 83183-3-PBS capture and 83183-4-PBS detection (validated in Cytometric bead array)

MP00148-2: 83183-2-PBS capture and 83183-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Indirect ELISA, WB Application Description
Tested Reactivity human
Immunogen KLF9 fusion protein Ag34411 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Kruppel-like factor 9
Synonyms GC-box-binding protein 1, BTE-binding protein 1, BTEB1, BTEB, Basic transcription element-binding protein 1
Calculated Molecular Weight27 kDa
GenBank Accession NumberBC074880
Gene Symbol KLF9
Gene ID (NCBI) 687
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13886
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C