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验证数据展示

  • WB analysis of mouse colon using 83228-1-RR (same clone as 83228-1-PBS)

    WB analysis of mouse colon using 83228-1-RR (same clone as 83228-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 83228-1-RR (SULT1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83228-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 83228-1-RR (same clone as 83228-1-PBS)

    IHC staining of mouse colon using 83228-1-RR (same clone as 83228-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83228-1-RR (SULT1B1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83228-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 83228-1-RR (same clone as 83228-1-PBS)

    IHC staining of mouse colon using 83228-1-RR (same clone as 83228-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83228-1-RR (SULT1B1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83228-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 83228-1-RR (same clone as 83228-1-PBS)

    FC experiment of A431 using 83228-1-RR (same clone as 83228-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug SULT1B1 Recombinant antibody (83228-1-RR, Clone:240119C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83228-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00184-1

    Cytometric bead array standard curve of MP00184-1

    Cytometric bead array standard curve of MP00184-1, SULT1B1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83228-1-PBS. Detection antibody: 83228-2-PBS. Standard: Ag9009. Range: 2.5-80 ng/mL.

  • Affinity and Kinetic Characterization of 83228-1-PBS

    Affinity and Kinetic Characterization of 83228-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 83228-1-PBS against Human SULT1B1 was performed. The affinity constant is 5.19 nM.

  • Affinity and Kinetic Characterization of 83228-1-RR

    Affinity and Kinetic Characterization of 83228-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83228-1-RR against Human SULT1B1 were performed. The affinity constant is 63.8 nM.

SULT1B1 Recombinant antibody, PBS Only (Capture)

SULT1B1 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83228-1-PBS

产品说明书

CloneNo. 240119C12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

SULT1B2, ST1B2, ST1B1, EC:2.8.2.1, 240119C12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83228-1-PBS targets SULT1B1 as part of a matched antibody pair:

MP00184-1: 83228-1-PBS capture and 83228-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 SULT1B1 fusion protein Ag9009 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sulfotransferase family, cytosolic, 1B, member 1
别名 SULT1B2, ST1B2, ST1B1, EC:2.8.2.1, 240119C12
计算分子量 296 aa, 35 kDa
观测分子量35 kDa
GenBank蛋白编号BC010895
基因名称 SULT1B1
Gene ID (NCBI) 27284
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43704
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Sulfotransferase 1B1 (SULT1B1) is expressed at highest levels throughout the human colon and small intestine but can also be found at moderate levels in human liver, kidney, and white blood cells (PMID: 28084139). Certain SULT1B1 SNPs may influence the activity of thyroid hormones and the mutagenicity of polycyclic aromatic hydrocarbons (PMID: 34107842).