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DGAT2 Recombinant antibody, PBS Only (Capture)

DGAT2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240171C6

Cat no : 83262-2-PBS

Print datasheet

Synonyms

EC:2.3.1.20, Diglyceride acyltransferase 2, Diacylglycerol O-acyltransferase 2, ARAT, Acyl-CoA retinol O-fatty-acyltransferase

验证数据展示

  • IF Staining of HepG2 using 83262-2-RR (same clone as 83262-2-PBS)

    IF Staining of HepG2 using 83262-2-RR (same clone as 83262-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DGAT2 antibody (83262-2-RR, Clone: 240171C6 ) at dilution of 1:150 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83262-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83262-2-RR (same clone as 83262-2-PBS)

    FC experiment of HeLa using 83262-2-RR (same clone as 83262-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Dgat2 Recombinant Antibody (83262-2-RR, Clone:240171C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83262-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00252-1

    Cytometric bead array standard curve of MP00252-1

    Cytometric bead array standard curve ofMP00252-1, DGAT2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83262-2-PBS. Detection antibody: 83262-1-PBS. Standard: Ag10719. Range: 3.13-100 ng/mL.

  • Cytometric bead array standard curve of MP00252-2

    Cytometric bead array standard curve of MP00252-2

    Cytometric bead array standard curve ofMP00252-2, DGAT2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83262-2-PBS. Detection antibody: 83262-3-PBS. Standard: Ag10719. Range: 3.13-100 ng/mL.

产品信息

83262-2-PBS targets DGAT2 as part of a matched antibody pair:

MP00252-1: 83262-2-PBS capture and 83262-1-PBS detection (validated in Cytometric bead array)

MP00252-2: 83262-2-PBS capture and 83262-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen DGAT2 fusion protein Ag10719 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name diacylglycerol O-acyltransferase homolog 2 (mouse)
Synonyms EC:2.3.1.20, Diglyceride acyltransferase 2, Diacylglycerol O-acyltransferase 2, ARAT, Acyl-CoA retinol O-fatty-acyltransferase
Calculated Molecular Weight 388 aa, 44 kDa
GenBank Accession NumberBC015234
Gene Symbol DGAT2
Gene ID (NCBI) 84649
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96PD7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C