验证数据展示
经过测试的应用
| Positive WB detected in | Ramos cells, Raji cells, THP-1 cells |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | THP-1 cells |
| Positive FC (Intra) detected in | HeLa cells, THP-1 cells |
Planning an IHC experiment? We recommend our IHCeasy DOCK8 Ready-To-Use IHC Kit. DOCK8 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83270-2-RR targets DOCK8 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | DOCK8 fusion protein Ag34585 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | dedicator of cytokinesis 8 |
| 别名 | MRD2, dedicator of cytokinesis 8, 230441A2 |
| 计算分子量 | 2031 aa, 231 kDa |
| 观测分子量 | 239 kDa |
| GenBank蛋白编号 | BC019102 |
| 基因名称 | DOCK8 |
| Gene ID (NCBI) | 81704 |
| RRID | AB_3670943 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q8NF50 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DOCK8 is a member of the DOCK family of proteins, which have a unique DRH2 domain enabling them to act as GEFs and so controlling a range of cellular processes in various signaling pathways (PMID: 12432077). The specific target of DOCK8 is Cell division control protein 42 homolog (Cdc42), a small GTPase that is involved in regulation of the cell cycle and forms a complex. DOCK8 also acts as a scaffold molecule in this complex that initiates actin polymerization via the Wiskott-Aldrich Syndrome protein (WASp) (PubMed: 28028151, PubMed: 22461490).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DOCK8 antibody 83270-2-RR | Download protocol |
| IHC protocol for DOCK8 antibody 83270-2-RR | Download protocol |
| IF protocol for DOCK8 antibody 83270-2-RR | Download protocol |
| FC protocol for DOCK8 antibody 83270-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |