CoraLite® Plus 488-conjugated DOCK8 Recombinant antibody
DOCK8 Uni-rAbTM Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
230441A2
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | THP-1 cells |
For other applications, we recommend the unconjugated version of this antibody, 83270-2-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-83270-2 targets DOCK8 in IF/ICC applications and shows reactivity with human samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human |
| Immunogen | DOCK8 fusion protein Ag34585 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | dedicator of cytokinesis 8 |
| Synonyms | |
| Calculated Molecular Weight | 2031 aa, 231 kDa |
| Observed Molecular Weight | 239 kDa |
| GenBank Accession Number | BC019102 |
| Gene Symbol | DOCK8 |
| Gene ID (NCBI) | 81704 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8NF50 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DOCK8 is a member of the DOCK family of proteins, which have a unique DRH2 domain enabling them to act as GEFs and so controlling a range of cellular processes in various signaling pathways (PMID: 12432077). The specific target of DOCK8 is Cell division control protein 42 homolog (Cdc42), a small GTPase that is involved in regulation of the cell cycle and forms a complex. DOCK8 also acts as a scaffold molecule in this complex that initiates actin polymerization via the Wiskott-Aldrich Syndrome protein (WASp) (PubMed: 28028151, PubMed: 22461490).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 DOCK8 antibody CL488-83270-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |