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DOCK8 Recombinant antibody

DOCK8 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230441A2

Cat no : 83270-2-RR

Print datasheet

Synonyms

MRD2, dedicator of cytokinesis 8, 230441A2

验证数据展示

  • WB analysis using 83270-2-RR

    WB analysis using 83270-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83270-2-RR (DOCK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 83270-2-RR

    IHC staining of mouse kidney using 83270-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83270-2-RR (DOCK8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of THP-1 using 83270-2-RR

    IF Staining of THP-1 using 83270-2-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using DOCK8 antibody (83270-2-RR, Clone: 230441A2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of THP-1 using 83270-2-RR

    FC experiment of THP-1 using 83270-2-RR

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug DOCK8 Recombinant antibody (83270-2-RR, Clone:230441A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HeLa using 83270-2-RR

    FC experiment of HeLa using 83270-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DOCK8 Recombinant antibody (83270-2-RR, Clone:230441A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83270-2-RR

    Affinity and Kinetic Characterization of 83270-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83270-2-RR against Human DOCK8 were performed. The affinity constant is 10.1 nM.

经过测试的应用

Positive WB detected inRamos cells, Raji cells, THP-1 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells
Positive FC (Intra) detected inHeLa cells, THP-1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83270-2-RR targets DOCK8 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen DOCK8 fusion protein Ag34585 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name dedicator of cytokinesis 8
Synonyms MRD2, dedicator of cytokinesis 8, 230441A2
Calculated Molecular Weight 2031 aa, 231 kDa
Observed Molecular Weight 239 kDa
GenBank Accession NumberBC019102
Gene Symbol DOCK8
Gene ID (NCBI) 81704
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8NF50
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DOCK8 is a member of the DOCK family of proteins, which have a unique DRH2 domain enabling them to act as GEFs and so controlling a range of cellular processes in various signaling pathways (PMID: 12432077). The specific target of DOCK8 is Cell division control protein 42 homolog (Cdc42), a small GTPase that is involved in regulation of the cell cycle and forms a complex. DOCK8 also acts as a scaffold molecule in this complex that initiates actin polymerization via the Wiskott-Aldrich Syndrome protein (WASp) (PubMed: 28028151, PubMed: 22461490).

实验方案

Product Specific Protocols
WB protocol for DOCK8 antibody 83270-2-RRDownload protocol
IHC protocol for DOCK8 antibody 83270-2-RRDownload protocol
IF protocol for DOCK8 antibody 83270-2-RRDownload protocol
FC protocol for DOCK8 antibody 83270-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols