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EPHB2 Recombinant antibody

EPHB2 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230111A5

Cat no : 83277-1-RR

Print datasheet

Synonyms

EK5, EC:2.7.10.1, DRT, Developmentally-regulated Eph-related tyrosine kinase, CAPB

验证数据展示

  • FC experiment of HepG2 using 83277-1-RR

    FC experiment of HepG2 using 83277-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug EPHB2 Recombinant antibody (83277-1-RR, Clone:230111A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 83277-1-RR

    WB analysis using 83277-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83277-1-RR (EPHB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHepG2 cells, U-251 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 83277-1-RR is EPHB2 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen EPHB2 fusion protein Ag28786 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name EPH receptor B2
Synonyms EK5, EC:2.7.10.1, DRT, Developmentally-regulated Eph-related tyrosine kinase, CAPB
Calculated Molecular Weight 987 aa, 108 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC018763
Gene Symbol EPHB2
Gene ID (NCBI) 2048
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP29323
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EPHB2 antibody 83277-1-RRDownload protocol
FC protocol for EPHB2 antibody 83277-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

tsRNA-GlyGCC promotes colorectal cancer progression and 5-FU resistance by regulating SPIB

Authors - Rong Xu
View Article