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EPHB2 Recombinant antibody, PBS Only

EPHB2 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230111A5

Cat no : 83277-1-PBS

Print datasheet

Synonyms

EK5, EC:2.7.10.1, DRT, Developmentally-regulated Eph-related tyrosine kinase, CAPB

验证数据展示

  • FC experiment of HepG2 using 83277-1-RR (same clone as 83277-1-PBS)

    FC experiment of HepG2 using 83277-1-RR (same clone as 83277-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug EPHB2 Recombinant antibody (83277-1-RR, Clone:230111A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83277-1-PBS in a different storage buffer formulation.

  • WB analysis using 83277-1-RR (same clone as 83277-1-PBS)

    WB analysis using 83277-1-RR (same clone as 83277-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83277-1-RR (EPHB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83277-1-PBS in a different storage buffer formulation.

产品信息

The immunogen of 83277-1-PBS is EPHB2 Fusion Protein expressed in E. coli.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human
Immunogen EPHB2 fusion protein Ag28786 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name EPH receptor B2
Synonyms EK5, EC:2.7.10.1, DRT, Developmentally-regulated Eph-related tyrosine kinase, CAPB
Calculated Molecular Weight 987 aa, 108 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC018763
Gene Symbol EPHB2
Gene ID (NCBI) 2048
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP29323
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C