验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, U-251 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
83277-1-RR targets EPHB2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | EPHB2 fusion protein Ag28786 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | EPH receptor B2 |
| 别名 | EK5, EC:2.7.10.1, DRT, Developmentally-regulated Eph-related tyrosine kinase, CAPB |
| 计算分子量 | 987 aa, 108 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | BC018763 |
| 基因名称 | EPHB2 |
| Gene ID (NCBI) | 2048 |
| RRID | AB_3670947 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P29323 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EPHB2 antibody 83277-1-RR | Download protocol |
| FC protocol for EPHB2 antibody 83277-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |