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Phospho-Histone H2A.X (Ser139) Recombinant antibody

Phospho-Histone H2A.X (Ser139) Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5N19

Cat no : 83307-2-RR

Print datasheet

Synonyms

H2AFX, Histone H2A, H2AX, H2A.X, H2A histone family, member X

验证数据展示

  • WB analysis using 83307-2-RR

    WB analysis using 83307-2-RR

    Non-treated Jurkat cells, and staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 83307-2-RR (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as loading control.

  • IHC staining of Jurkat using 83307-2-RR

    IHC staining of Jurkat using 83307-2-RR

    Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 83307-2-RR (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with H2AX antibody included
  • IF Staining of HeLa using 83307-2-RR

    IF Staining of HeLa using 83307-2-RR

    Immunofluorescent analysis of (4% PFA) fixed UV treated HeLa cells using Phospho-Histone H2A.X (Ser139) antibody (83307-2-RR, Clone: 5N19 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inStaurosporine treated Jurkat cells
Positive IHC detected inJurkat cells
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inUV treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy H2AX Ready-To-Use IHC Kit. H2AX primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

83307-2-RR targets Phospho-Histone H2A.X (Ser139) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name H2A histone family, member X
Synonyms H2AFX, Histone H2A, H2AX, H2A.X, H2A histone family, member X
Calculated Molecular Weight 15 kDa
Observed Molecular Weight15 kDa
GenBank Accession NumberBC013416
Gene Symbol Histone H2A.X
Gene ID (NCBI) 3014
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP16104
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The histone variant H2AX is a major component of the DNA damage response (DDR), especially functioning in amplifying DNA damage signals. In response to DNA double-strand breaks (DSBs), H2AX is instantaneously phosphorylated at Ser139 (a form called cH2AX) by the kinases ATM and ATR. The phosphorylation of H2AX at Ser139, resulting in the formation of gamma-H2AX puncta in the nuclei, is an early event in the cellular response to DNA damage. Therefore, phospho-Histone H2A. X (Ser139) is also known as γH2AX. The phosphorylation site of H2AX, Ser139, has also been described as Ser140 in other literature, and they recognize the same amino acid site. (PMID: 22908299, PMID: 30106130, PMID:22941631)

实验方案

Product Specific Protocols
WB protocol for Phospho-Histone H2A.X (Ser139) antibody 83307-2-RRDownload protocol
IHC protocol for Phospho-Histone H2A.X (Ser139) antibody 83307-2-RRDownload protocol
IF protocol for Phospho-Histone H2A.X (Ser139) antibody 83307-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Transl Oncol

PiR-hsa-23533 promotes malignancy in head and neck squamous cell carcinoma via USP7

Authors - Hanlin Hu
View Article