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验证数据展示

  • WB analysis using 83307-2-RR (same clone as 83307-2-PBS)

    WB analysis using 83307-2-RR (same clone as 83307-2-PBS)

    Non-treated Jurkat cells, and staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 83307-2-RR (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 83307-2-PBS in a different storage buffer formulation.

  • IHC staining of Jurkat using 83307-2-RR (same clone as 83307-2-PBS)

    IHC staining of Jurkat using 83307-2-RR (same clone as 83307-2-PBS)

    Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 83307-2-RR (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83307-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with H2AX antibody included
  • IHC staining of Jurkat using 83307-2-RR (same clone as 83307-2-PBS)

    IHC staining of Jurkat using 83307-2-RR (same clone as 83307-2-PBS)

    Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 83307-2-RR (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83307-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with H2AX antibody included
  • IF Staining of HeLa using 83307-2-RR (same clone as 83307-2-PBS)

    IF Staining of HeLa using 83307-2-RR (same clone as 83307-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed UV treated HeLa cells using Phospho-Histone H2A.X (Ser139) antibody (83307-2-RR, Clone: 5N19 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83307-2-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 83307-2-RR (same clone as 83307-2-PBS)

    FC experiment of Jurkat using 83307-2-RR (same clone as 83307-2-PBS)

    1x10^6 Jurkat cells untreated (dashed lines) or treated with Staurosporine which intracellularly stained with 0.06 ug Phospho-Histone H2A.X (Ser139) Recombinant antibody (83307-2-RR, Clone:5N19) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 83307-2-PBS in a different storage buffer formulation.

Phospho-Histone H2A.X (Ser139) Recombinant antibody, PBS Only

Phospho-Histone H2A.X (Ser139) Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83307-2-PBS

产品说明书

CloneNo. 5N19

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

Histone H2A.X (Ser139), p Histone H2A.X, p Histone H2A.X (Ser139), p Histone H2A.X Ser139, p-Histone H2A.X

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy H2AX Ready-To-Use IHC Kit. H2AX primary antibody included.

产品信息

83307-2-PBS targets Phospho-Histone H2A.X (Ser139) in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 H2A histone family, member X
别名 Histone H2A.X (Ser139), p Histone H2A.X, p Histone H2A.X (Ser139), p Histone H2A.X Ser139, p-Histone H2A.X
计算分子量 15 kDa
观测分子量15 kDa
GenBank蛋白编号BC013416
基因名称 Histone H2A.X
Gene ID (NCBI) 3014
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP16104
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The histone variant H2AX is a major component of the DNA damage response (DDR), especially functioning in amplifying DNA damage signals. In response to DNA double-strand breaks (DSBs), H2AX is instantaneously phosphorylated at Ser139 (a form called cH2AX) by the kinases ATM and ATR. The phosphorylation of H2AX at Ser139, resulting in the formation of gamma-H2AX puncta in the nuclei, is an early event in the cellular response to DNA damage. Therefore, phospho-Histone H2A. X (Ser139) is also known as γH2AX. The phosphorylation site of H2AX, Ser139, has also been described as Ser140 in other literature, and they recognize the same amino acid site. (PMID: 22908299, PMID: 30106130, PMID:22941631)