Phospho-Histone H2A.X (Ser139) Recombinant antibody, PBS Only

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5N19

Cat No : 83307-2-PBS

Synonyms

H2A histone family, member X, H2A.X, H2A/X, H2AFX, H2AX, Histone H2A.x

WB analysis using 83307-2-RR (same clone as 83307-2-PBS)
Non-treated Jurkat cells, and staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 83307-2-RR (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 83307-2-PBS in a different storage buffer formulation.
×
Non-treated Jurkat cells, and staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 83307-2-RR (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 83307-2-PBS in a different storage buffer formulation.

Size

Planning an IHC experiment? We recommend our IHCeasy H2AX Ready-To-Use IHC Kit. H2AX primary antibody included.

83307-2-PBS targets Phospho-Histone H2A.X (Ser139) in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications	WB, Indirect ELISA Application Description
Tested Reactivity	Human
Immunogen	Peptide 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Full Name	H2A histone family, member X
Synonyms	H2A histone family, member X, H2A.X, H2A/X, H2AFX, H2AX, Histone H2A.x
Calculated Molecular Weight	15 kDa
GenBank Accession Number	BC013416
Gene Symbol	H2AFX
Gene ID (NCBI)	3014
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	P16104
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.