验证数据展示
经过测试的应用
| Positive WB detected in | Staurosporine treated Jurkat cells |
| Positive IHC detected in | Jurkat cells Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | UV treated HeLa cells |
| Positive FC (Intra) detected in | Staurosporine treated Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
83307-2-RR targets Phospho-Histone H2A.X (Ser139) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | H2A histone family, member X |
| 别名 | H2A.X Ser139, H2A.X (Ser139), Histone H2A.X (Ser139), Histone H2A.X Ser139, p Histone H2A.X |
| 计算分子量 | 15 kDa |
| 观测分子量 | 15 kDa |
| GenBank蛋白编号 | BC013416 |
| 基因名称 | Histone H2A.X |
| Gene ID (NCBI) | 3014 |
| RRID | AB_3670974 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P16104 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The histone variant H2AX is a major component of the DNA damage response (DDR), especially functioning in amplifying DNA damage signals. In response to DNA double-strand breaks (DSBs), H2AX is instantaneously phosphorylated at Ser139 (a form called cH2AX) by the kinases ATM and ATR. The phosphorylation of H2AX at Ser139, resulting in the formation of gamma-H2AX puncta in the nuclei, is an early event in the cellular response to DNA damage. Therefore, phospho-Histone H2A. X (Ser139) is also known as γH2AX. The phosphorylation site of H2AX, Ser139, has also been described as Ser140 in other literature, and they recognize the same amino acid site. (PMID: 22908299, PMID: 30106130, PMID:22941631)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-Histone H2A.X (Ser139) antibody 83307-2-RR | Download protocol |
| IHC protocol for Phospho-Histone H2A.X (Ser139) antibody 83307-2-RR | Download protocol |
| IF protocol for Phospho-Histone H2A.X (Ser139) antibody 83307-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |