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Bcl-XL Recombinant antibody

Bcl-XL Recombinant Antibody for WB, IF/ICC, ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

1E8

Cat No : 83330-1-RR

Print datasheet

Synonyms

Apoptosis regulator Bcl X, BCL2L1, DKFZp781P2092

验证数据展示

  • WB analysis using 83330-1-RR

    WB analysis using 83330-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83330-1-RR (Bcl-xL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83330-1-RR

    IF Staining of HeLa using 83330-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Bcl-XL antibody (83330-1-RR, Clone: 1E8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 83330-1-RR

    FC experiment of HeLa using 83330-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Bcl-XL Recombinant antibody (83330-1-RR, Clone:1E8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity experiments of 83330-1-RR

    Affinity experiments of 83330-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83330-1-RR against Human Bcl-XL were performed. The affinity constant is 0.792 nM.

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, Ramos cells, K-562 cells, RAW 264.7 cells, C6 cells, Jurkat cells
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy BCL2L1 Ready-To-Use IHC Kit. BCL2L1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:125-1:500
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83330-1-RR is Bcl-XL Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA, FC (Intra) Application Description
Tested Reactivity Human, mouse, rat
Immunogen Bcl-XL fusion protein Ag25604 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name BCL2-like 1
Synonyms Apoptosis regulator Bcl X, BCL2L1, DKFZp781P2092
Calculated Molecular Weight 26 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC019307
Gene Symbol Bcl-XL
Gene ID (NCBI) 598
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ07817
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L), Bcl-X(s), and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-X(L) isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can form heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-X(s) isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. Bcl-X(beta) is a 227 amino acid protein.

实验方案

Product Specific Protocols
WB protocol for Bcl-XL antibody 83330-1-RRDownload protocol
IF protocol for Bcl-XL antibody 83330-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols