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Host
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验证数据展示

  • WB analysis using 83330-1-RR (same clone as 83330-1-PBS)

    WB analysis using 83330-1-RR (same clone as 83330-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83330-1-RR (Bcl-xL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83330-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83330-1-RR (same clone as 83330-1-PBS)

    IF Staining of HeLa using 83330-1-RR (same clone as 83330-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Bcl-XL antibody (83330-1-RR, Clone: 1E8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83330-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83330-1-RR (same clone as 83330-1-PBS)

    FC experiment of HeLa using 83330-1-RR (same clone as 83330-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Bcl-XL Recombinant antibody (83330-1-RR, Clone:1E8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83330-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83330-1-RR

    Affinity and Kinetic Characterization of 83330-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83330-1-RR against Human Bcl-XL were performed. The affinity constant is 0.792 nM.

Bcl-XL Recombinant antibody, PBS Only

Bcl-XL Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83330-1-PBS

产品说明书

CloneNo. 1E8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

BCL2L1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X, Apoptosis regulator Bcl X

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy BCL2L1 Ready-To-Use IHC Kit. BCL2L1 primary antibody included.

产品信息

83330-1-PBS targets Bcl-XL in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Bcl-XL fusion protein Ag25604 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 BCL2-like 1
别名 BCL2L1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X, Apoptosis regulator Bcl X
计算分子量 26 kDa
GenBank蛋白编号BC019307
基因名称 Bcl-XL
Gene ID (NCBI) 598
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ07817
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C