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Bcl-xL Recombinant antibody, PBS Only

Bcl-xL Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E8

Cat no : 83330-1-PBS

Print datasheet

Synonyms

Apoptosis regulator Bcl X, BCL2L1, DKFZp781P2092

验证数据展示

  • WB analysis using 83330-1-RR (same clone as 83330-1-PBS)

    WB analysis using 83330-1-RR (same clone as 83330-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83330-1-RR (Bcl-xL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83330-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83330-1-RR (same clone as 83330-1-PBS)

    IF Staining of HeLa using 83330-1-RR (same clone as 83330-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Bcl-XL antibody (83330-1-RR, Clone: 1E8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83330-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83330-1-RR (same clone as 83330-1-PBS)

    FC experiment of HeLa using 83330-1-RR (same clone as 83330-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Bcl-XL Recombinant antibody (83330-1-RR, Clone:1E8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83330-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83330-1-PBS

    Affinity and Kinetic Characterization of 83330-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 83330-1-RR against Human Bcl-XL were performed. The affinity constant is 0.792 nM.

Planning an IHC experiment? We recommend our IHCeasy BCL2L1 Ready-To-Use IHC Kit. BCL2L1 primary antibody included.

产品信息

The immunogen of 83330-1-PBS is Bcl-xL Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity Human
Immunogen Bcl-xL fusion protein Ag25604 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name BCL2-like 1
Synonyms Apoptosis regulator Bcl X, BCL2L1, DKFZp781P2092
Calculated Molecular Weight 26 kDa
GenBank Accession NumberBC019307
Gene Symbol Bcl-XL
Gene ID (NCBI) 598
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ07817
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C