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验证数据展示

  • FC experiment of HeLa using CL488-83330

    FC experiment of HeLa using CL488-83330

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Bcl-XL Recombinant Antibody (CL488-83330, Clone:1E8)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated Bcl-XL Recombinant antibody

Bcl-XL Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. CL488-83330

Print datasheet

CloneNo. 1E8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Synonyms

BCL2L1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X, Apoptosis regulator Bcl X

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy BCL2L1 Ready-To-Use IHC Kit. BCL2L1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83330-1-PBS

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83330 targets Bcl-XL in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Bcl-XL fusion protein Ag25604 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name BCL2-like 1
Synonyms BCL2L1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X, Apoptosis regulator Bcl X
Calculated Molecular Weight 26 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC019307
Gene Symbol Bcl-XL
Gene ID (NCBI) 598
RRIDAB_3673239
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07817
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L), Bcl-X(s), and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-X(L) isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can form heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-X(s) isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. Bcl-X(beta) is a 227 amino acid protein.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Bcl-XL antibody CL488-83330Download protocol
Standard Protocols
Click here to view our Standard Protocols