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RAB13 Recombinant antibody, PBS Only (Capture)

RAB13 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240216D10

Cat no : 83336-2-PBS

Print datasheet

Synonyms

GIG4, Ras-related protein Rab-13, Ras related protein Rab 13, Cell growth-inhibiting gene 4 protein, Cell growth inhibiting gene 4 protein

验证数据展示

  • WB analysis using 83336-2-RR (same clone as 83336-2-PBS)

    WB analysis using 83336-2-RR (same clone as 83336-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83336-2-RR (RAB13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83336-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83336-2-RR (same clone as 83336-2-PBS)

    IF Staining of HeLa using 83336-2-RR (same clone as 83336-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB13 antibody (83336-2-RR, Clone: 240216D10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83336-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83336-2-RR (same clone as 83336-2-PBS)

    IF Staining of HeLa using 83336-2-RR (same clone as 83336-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB13 antibody (83336-2-RR, Clone: 240216D10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83336-2-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 83336-2-RR (same clone as 83336-2-PBS)

    FC experiment of K-562 using 83336-2-RR (same clone as 83336-2-PBS)

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug RAB13 Recombinant antibody (83336-2-RR, Clone:240216D10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83336-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00354-1

    Cytometric bead array standard curve of MP00354-1

    Cytometric bead array standard curve of MP00354-1, RAB13 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83336-2-PBS. Detection antibody: 83336-1-PBS. Standard: Ag34898. Range: 0.156-20 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy RAB13 Ready-To-Use IHC Kit. RAB13 primary antibody included.

产品信息

83336-2-PBS targets RAB13 as part of a matched antibody pair:

MP00354-1: 83336-2-PBS capture and 83336-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen RAB13 fusion protein Ag34898 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RAB13, member RAS oncogene family
Synonyms GIG4, Ras-related protein Rab-13, Ras related protein Rab 13, Cell growth-inhibiting gene 4 protein, Cell growth inhibiting gene 4 protein
Calculated Molecular Weight 203 aa, 23 kDa
Observed Molecular Weight23 kDa
GenBank Accession NumberBC000799
Gene Symbol RAB13
Gene ID (NCBI) 5872
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51153
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAB13 (Ras-related protein Rab-13) is also named as GIG4. Rab13 is a member of the Rab GTPase family. RAB13 is highly expressed in malignant cells. Rab13 was highly expressed in BCSC of triple-negative breast cancer (TNBC) (PMID: 35395074). RAB13 is involved in a number of processes in LIHC, including MTORC1 signaling, MYC targets v1, G2M checkpoint, MITOTIC spindle, DNA repair, P53 pathway, glycolysis, PI3K-AKT-MTOR signaling, etc (PMID: 37803063). RAB13 enhances its circulation to the plasma membrane to promote breast cancer invasion. By influencing the tumor microenvironment, RAB13 maintains breast cancer stem cells (PMID: 37803063).