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CRIP1 Recombinant antibody, PBS Only (Capture/Detector)

CRIP1 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240176G2

Cat no : 83340-3-PBS

Print datasheet

Synonyms

CRP1, CRP 1, CRIP 1, CRIP, CRHP

验证数据展示

  • WB analysis using 83340-3-RR (same clone as 83340-3-PBS)

    WB analysis using 83340-3-RR (same clone as 83340-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83340-3-RR (CRIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83340-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00382-3

    Sandwich ELISA standard curve of MP00382-3

    Sandwich ELISA standard curve of MP00382-3, Human CRIP1 Recombinant Matched Antibody Pair - PBS only. 83340-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7588. 83340-3-PBS was HRP conjugated as the detection antibody. Range: 46.88-3000 pg/mL.

  • Sample test of MP00382-3

    Sample test of MP00382-3

    The mean CRIP1 concentration was determined to be 3,364.0 pg/mL in PC-3 cell extract based on a 3.0 mg/mL extract load and 3,015.0 pg/mL in HeLa cell extract based on a 3.0 mg/mL extract load.

  • IHC staining of human colon using 83340-3-RR (same clone as 83340-3-PBS)

    IHC staining of human colon using 83340-3-RR (same clone as 83340-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 83340-3-RR (CRIP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83340-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00382-1

    Cytometric bead array standard curve of MP00382-1

    Cytometric bead array standard curve of MP00382-1, CRIP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83340-3-PBS. Detection antibody: 83340-2-PBS. Standard: Ag7588. Range: 0.156-20 ng/mL.

  • Cytometric bead array standard curve of MP00382-2

    Cytometric bead array standard curve of MP00382-2

    Cytometric bead array standard curve of MP00382-2, CRIP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83340-1-PBS. Detection antibody: 83340-3-PBS. Standard: Ag7588. Range: 0.156-20 ng/mL.

  • Affinity and Kinetic Characterization of 83340-3-RR

    Affinity and Kinetic Characterization of 83340-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83340-3-RR against Human CRIP1 were performed. The affinity constant is 0.784 nM.

产品信息

83340-3-PBS targets CRIP1 as part of a matched antibody pair:

MP00382-1: 83340-3-PBS capture and 83340-2-PBS detection (validated in Cytometric bead array)

MP00382-2: 83340-1-PBS capture and 83340-3-PBS detection (validated in Cytometric bead array)

MP00382-3: 83340-4-PBS capture and 83340-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human, mouse
Immunogen CRIP1 fusion protein Ag7588 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cysteine-rich protein 1 (intestinal)
Synonyms CRP1, CRP 1, CRIP 1, CRIP, CRHP
Calculated Molecular Weight 9 kDa
Observed Molecular Weight 8-9 kDa
GenBank Accession NumberBC002738
Gene Symbol CRIP1
Gene ID (NCBI) 1396
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50238
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CRIP1 also known as CRIP, CRP1, belongs to the LIM/double zinc finger protein family. CRIP1 has a unique double zinc finger motif, which is mainly expressed in the intestine, and may be involved in intestinal zinc transport (PMID: 31312368, 1946385). CRIP1 is overexpressed in immune cells of the epithelium and may play an important role in gut immunity(PMID: 27836662).