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验证数据展示

  • FC experiment of A549 using 83376-3-RR (same clone as 83376-3-PBS)

    FC experiment of A549 using 83376-3-RR (same clone as 83376-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Neil1 Recombinant Antibody (83376-3-RR, Clone:240322B7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83376-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 83376-3-RR (same clone as 83376-3-PBS)

    IHC staining of mouse spleen using 83376-3-RR (same clone as 83376-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83376-3-RR (NEIL1 antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83376-3-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83376-3-RR (same clone as 83376-3-PBS)

    IF Staining of HepG2 using 83376-3-RR (same clone as 83376-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NEIL1 antibody (83376-3-RR, Clone: 240322B7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83376-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00380-2

    Cytometric bead array standard curve of MP00380-2

    Cytometric bead array standard curve of MP00380-2, NEIL1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83376-4-PBS. Detection antibody: 83376-3-PBS. Standard: Ag2793. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83376-3-PBS

    Affinity and Kinetic Characterization of 83376-3-PBS

    Biolayer interferometry (BLl) kinetic assay of 83376-3-PBS against Human NEIL1 was performed. The affinity constant is below 1 pM.

NEIL1 Recombinant antibody, PBS Only (Detector)

NEIL1 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83376-3-PBS

产品说明书

CloneNo. 240322B7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

NEI L1, Endonuclease VIII-like 1, Endonuclease 8-like 1, EC:3.2.2.-, DNA-(apurinic or apyrimidinic site) lyase Neil1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83376-3-PBS targets NEIL1 as part of a matched antibody pair:

MP00380-2: 83376-4-PBS capture and 83376-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 NEIL1 fusion protein Ag2793 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nei endonuclease VIII-like 1 (E. coli)
别名 NEI L1, Endonuclease VIII-like 1, Endonuclease 8-like 1, EC:3.2.2.-, DNA-(apurinic or apyrimidinic site) lyase Neil1
计算分子量 390 aa, 44 kDa
GenBank蛋白编号BC010876
基因名称 NEIL1
Gene ID (NCBI) 79661
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96FI4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C