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NEIL1 Recombinant antibody, PBS Only (Detector)

NEIL1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240322B7

Cat no : 83376-3-PBS

Print datasheet

Synonyms

NEI L1, Endonuclease VIII-like 1, Endonuclease 8-like 1, EC:3.2.2.-, DNA-(apurinic or apyrimidinic site) lyase Neil1

验证数据展示

  • FC experiment of A549 using 83376-3-RR (same clone as 83376-3-PBS)

    FC experiment of A549 using 83376-3-RR (same clone as 83376-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Neil1 Recombinant Antibody (83376-3-RR, Clone:240322B7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83376-3-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83376-3-RR (same clone as 83376-3-PBS)

    IF Staining of HepG2 using 83376-3-RR (same clone as 83376-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NEIL1 antibody (83376-3-RR, Clone: 240322B7 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83376-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00380-2

    Cytometric bead array standard curve of MP00380-2

    Cytometric bead array standard curve of MP00380-2, NEIL1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83376-4-PBS. Detection antibody: 83376-3-PBS. Standard: Ag2793. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83376-3-RR

    Affinity and Kinetic Characterization of 83376-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83376-3-RR against Human NEIL1 were performed. The affinity constant is below 1 pM.

产品信息

83376-3-PBS targets NEIL1 as part of a matched antibody pair:

MP00380-2: 83376-4-PBS capture and 83376-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen NEIL1 fusion protein Ag2793 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nei endonuclease VIII-like 1 (E. coli)
Synonyms NEI L1, Endonuclease VIII-like 1, Endonuclease 8-like 1, EC:3.2.2.-, DNA-(apurinic or apyrimidinic site) lyase Neil1
Calculated Molecular Weight 390 aa, 44 kDa
GenBank Accession NumberBC010876
Gene Symbol NEIL1
Gene ID (NCBI) 79661
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96FI4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C