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Conjugate
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Conjugate
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SYCP1 Recombinant antibody

SYCP1 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240033E3

Cat No : 83382-1-RR

Print datasheet

Synonyms

SYCP 1, SCP-1, SCP1, SCP 1, CT8

验证数据展示

  • WB analysis of mouse testis using 83382-1-RR

    WB analysis of mouse testis using 83382-1-RR

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 83382-1-RR (SYCP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of MCF-7 using 83382-1-RR

    FC experiment of MCF-7 using 83382-1-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Sycp1 Recombinant Antibody (83382-1-RR, Clone:240033E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83382-1-RR

    Affinity and Kinetic Characterization of 83382-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83382-1-RR against Human SYCP1 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inmouse testis tissue
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83382-1-RR is SYCP1 Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen SYCP1 fusion protein Ag16005 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name synaptonemal complex protein 1
Synonyms SYCP 1, SCP-1, SCP1, SCP 1, CT8
Calculated Molecular Weight 976 aa, 114 kDa
Observed Molecular Weight115-120 kDa
GenBank Accession NumberBC126266
Gene Symbol SYCP1
Gene ID (NCBI) 6847
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ15431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SYCP1 antibody 83382-1-RRDownload protocol
FC protocol for SYCP1 antibody 83382-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols