SYCP1 Recombinant antibody, PBS Only

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240033E3

Cat No : 83382-1-PBS

Synonyms

SYCP 1, SCP-1, SCP1, SCP 1, CT8

WB analysis of mouse testis using 83382-1-RR (same clone as 83382-1-PBS)
mouse testis tissue were subjected to SDS PAGE followed by western blot with 83382-1-RR (SYCP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83382-1-PBS in a different storage buffer formulation.
FC experiment of MCF-7 using 83382-1-RR (same clone as 83382-1-PBS)
1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Sycp1 Recombinant Antibody (83382-1-RR, Clone:240033E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83382-1-PBS in a different storage buffer formulation.
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1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Sycp1 Recombinant Antibody (83382-1-RR, Clone:240033E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83382-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 83382-1-RR
Biolayer interferometry (BLl) kinetic assays of 83382-1-RR against Human SYCP1 were performed. The affinity constant is below 1 pM.

Size

The immunogen of 83382-1-PBS is SYCP1 Fusion Protein expressed in E. coli.

Tested Applications	WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity	human, mouse
Immunogen	SYCP1 fusion protein Ag16005 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Full Name	synaptonemal complex protein 1
Synonyms	SYCP 1, SCP-1, SCP1, SCP 1, CT8
Calculated Molecular Weight	976 aa, 114 kDa
Observed Molecular Weight	115-120 kDa
GenBank Accession Number	BC126266
Gene Symbol	SYCP1
Gene ID (NCBI)	6847
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	Q15431
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C