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验证数据展示

  • IF Staining of THP-1 using 83384-2-RR

    IF Staining of THP-1 using 83384-2-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using NLRC5 antibody (83384-2-RR, Clone: 240338D8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of THP-1 using 83384-2-RR

    FC experiment of THP-1 using 83384-2-RR

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug NLRC5 Recombinant antibody (83384-2-RR, Clone:240338D8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83384-2-RR

    Affinity and Kinetic Characterization of 83384-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83384-2-RR against Human NLRC5 were performed. The affinity constant is below 1 pM.

NLRC5 Recombinant antibody

NLRC5 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83384-2-RR

产品说明书

CloneNo. 240338D8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

NOD4, NOD27, CLR16.1, Caterpiller protein 16.1, 240338D8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inTHP-1 cells
Positive FC (Intra) detected inTHP-1 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83384-2-RR targets NLRC5 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 NLRC5 fusion protein Ag34508 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NLR family, CARD domain containing 5
别名 NOD4, NOD27, CLR16.1, Caterpiller protein 16.1, 240338D8
计算分子量204 kDa
GenBank蛋白编号BC063566
基因名称 NLRC5
Gene ID (NCBI) 84166
RRIDAB_3671040
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ86WI3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The nucleotide-binding domain leucine-rich repeat containing (NLR) family of proteins is mainly involved in microbial pathogen recognition, inflammatory responses, and cell death. NLRC5, the largest member of the NLR family, is currently receiving an increasing level of attention. NLRC5 plays a role in cytokine response and antiviral immunity through its inhibition of NF-kappa-B activation and negative regulation of type I interferon signaling pathways.

实验方案

Product Specific Protocols
IF protocol for NLRC5 antibody 83384-2-RRDownload protocol
FC protocol for NLRC5 antibody 83384-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols