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MPHOSPH9 Recombinant antibody

MPHOSPH9 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240182D6

Cat no : 83397-5-RR

Print datasheet

Synonyms

MPP9, MPP 9, M-phase phosphoprotein 9, 240182D6

验证数据展示

  • WB analysis using 83397-5-RR

    WB analysis using 83397-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83397-5-RR (MPHOSPH9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83397-5-RR

    WB analysis using 83397-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83397-5-RR (MPHOSPH9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 83397-5-RR

    FC experiment of A431 using 83397-5-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MPHOSPH9 Recombinant antibody (83397-5-RR, Clone:240182D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83397-5-RR

    Affinity and Kinetic Characterization of 83397-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83397-5-RR against Human MPHOSPH9 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, rat brain tissue, NIH/3T3 cells, PC-12 cells, SH-SY5Y cells, mouse brain tissue
Positive FC (Intra) detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83397-5-RR is MPHOSPH9 Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MPHOSPH9 fusion protein Ag22228 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name M-phase phosphoprotein 9
Synonyms MPP9, MPP 9, M-phase phosphoprotein 9, 240182D6
Calculated Molecular Weight 1031 aa, 116 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC130440
Gene Symbol MPHOSPH9
Gene ID (NCBI) 10198
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ99550
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MPHOSPH9, also known as MPP9, is a protein phosphorylated during mitosis. MPHOSPH9 negatively regulates cilia formation by recruiting the CP110-CEP97 complex at the distal end of the mother centriole in ciliary cells. MPHOSPH9 is a centrosome component that localizes to the distal and proximal ends of two centrioles(PMID: 30375385).

实验方案

Product Specific Protocols
WB protocol for MPHOSPH9 antibody 83397-5-RRDownload protocol
FC protocol for MPHOSPH9 antibody 83397-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols