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验证数据展示

  • WB analysis using 83401-4-RR (same clone as 83401-4-PBS)

    WB analysis using 83401-4-RR (same clone as 83401-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83401-4-RR (TSSK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83401-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 83401-4-RR (same clone as 83401-4-PBS)

    IHC staining of mouse testis using 83401-4-RR (same clone as 83401-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83401-4-RR (TSSK4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83401-4-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 83401-4-RR (same clone as 83401-4-PBS)

    IF Staining of mouse testis using 83401-4-RR (same clone as 83401-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using TSSK4 antibody (83401-4-RR, Clone: 240373F10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83401-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00436-2

    Cytometric bead array standard curve of MP00436-2

    Cytometric bead array standard curve of MP00436-2, TSSK4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83401-1-PBS. Detection antibody: 83401-4-PBS. Standard: Ag16205. Range: 6.25-100 ng/mL.

  • Cytometric bead array standard curve of MP00436-3

    Cytometric bead array standard curve of MP00436-3

    Cytometric bead array standard curve of MP00436-3, TSSK4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83401-2-PBS. Detection antibody: 83401-4-PBS. Standard: Ag16205. Range: 6.25-100 ng/mL.

  • Affinity and Kinetic Characterization of 83401-4-RR

    Affinity and Kinetic Characterization of 83401-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83401-4-RR against Human TSSK4 were performed. The affinity constant is 5.41 nM.

TSSK4 Recombinant antibody, PBS Only (Detector)

TSSK4 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 83401-4-PBS

产品说明书

CloneNo. 240373F10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

别名

testis serine kinase 4, STK22E, Serine/threonine-protein kinase 22E, EC:2.7.11.1, C14orf20

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83401-4-PBS targets TSSK4 as part of a matched antibody pair:

MP00436-2: 83401-1-PBS capture and 83401-4-PBS detection (validated in Cytometric bead array)

MP00436-3: 83401-2-PBS capture and 83401-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TSSK4 fusion protein Ag16205 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 testis-specific serine kinase 4
别名 testis serine kinase 4, STK22E, Serine/threonine-protein kinase 22E, EC:2.7.11.1, C14orf20
计算分子量 338 aa, 37 kDa
观测分子量 38-45 kDa
GenBank蛋白编号BC111088
基因名称 TSSK4
Gene ID (NCBI) 283629
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ6SA08
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TSSK4 (Testis-specific serine/threonine-protein kinase 4), testis-specific serine/threonine protein kinase 4, is a member of the testis-specific serine/threonine protein kinase family. It has autophosphorylation activity and self-binding properties; it can interact with and phosphorylate other proteins, e.g., it can bind to and phosphorylate Odf2. It's reported that TSSK4 plays an important role in spermatogenesis, TSSK4 knockout male mice develop reduced sperm motility and reduced fertility. In addition, TSSK4 regulates lung fibrosis by promoting the loss of alveolar epithelial type II cells through the HSP90-AKT signaling pathway.