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TRNP1 Recombinant antibody

TRNP1 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240331B6

Cat no : 83410-4-RR

Print datasheet

Synonyms

TRNP, TNRP, TMF-regulated nuclear protein 1, C1orf225, 240331B6

验证数据展示

  • IF Staining of HeLa using 83410-4-RR

    IF Staining of HeLa using 83410-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRNP1 antibody (83410-4-RR, Clone: 240331B6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83410-4-RR

    FC experiment of HeLa using 83410-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TRNP1 Recombinant antibody (83410-4-RR, Clone:240331B6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83410-4-RR

    Affinity and Kinetic Characterization of 83410-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83410-4-RR against Human TRNP1 were performed. The affinity constant is 2.5 nM.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83410-4-RR is TRNP1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen TRNP1 fusion protein Ag22504 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name TMF1-regulated nuclear protein 1
Synonyms TRNP, TNRP, TMF-regulated nuclear protein 1, C1orf225, 240331B6
Calculated Molecular Weight 227 aa, 23 kDa
GenBank Accession NumberBC069216
Gene Symbol TRNP1
Gene ID (NCBI) 388610
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ6NT89
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRNP1 (TMF-regulated nuclear protein 1), also called C1orf225, is a DNA-binding factor that regulates the expression of a subset of genes and plays a key role in tangential, radial, and lateral expansion of the brain neocortex (PMID: 27264089; 23622239; 36947129). It has been shown to exert potent roles in neural development affecting neural stem cell self-renewal and brain folding (PMID: 32627867). It may also control the proliferation rate of cells by regulating their progression through key cell-cycle transition points.

实验方案

Product Specific Protocols
IF protocol for TRNP1 antibody 83410-4-RRDownload protocol
FC protocol for TRNP1 antibody 83410-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols