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KIAA1715 Recombinant antibody

KIAA1715 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240211C7

Cat no : 83418-4-RR

Print datasheet

Synonyms

LNP, Ul, Protein lunapark, LNPK, ER junction formation factor lunapark

验证数据展示

  • WB analysis using 83418-4-RR

    WB analysis using 83418-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83418-4-RR (KIAA1715 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 83418-4-RR

    IHC staining of mouse cerebellum using 83418-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83418-4-RR (KIAA1715 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of U-251 using 83418-4-RR

    FC experiment of U-251 using 83418-4-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug KIAA1715 Recombinant antibody (83418-4-RR, Clone:240211C7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83418-4-RR

    Affinity and Kinetic Characterization of 83418-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83418-4-RR against Human KIAA1715 were performed. The affinity constant is 13.1 nM.

经过测试的应用

Positive WB detected inmouse testis tissue, mouse kidney tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inU-251 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83418-4-RR targets KIAA1715 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen KIAA1715 fusion protein Ag34669 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name KIAA1715
Synonyms LNP, Ul, Protein lunapark, LNPK, ER junction formation factor lunapark
Observed Molecular Weight51 kDa
GenBank Accession NumberBC105132
Gene Symbol KIAA1715
Gene ID (NCBI) 80856
RRIDAB_3671066
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9C0E8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIAA1715, also known as LNPK, is a member of the conserved Lunapark protein family. KIAA1715 is an endoplasmic reticulum (ER) membrane protein consisting of an N-terminal domain important for N-myristoylation and ER targeting, two transmembrane domains and a unique zinc finger domain. It has 4 isoforms produced by alternative splicing and acts mainly as a stabilizer of negative membrane curvature (PMID: 27619977).

实验方案

Product Specific Protocols
WB protocol for KIAA1715 antibody 83418-4-RRDownload protocol
IHC protocol for KIAA1715 antibody 83418-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols