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ESR2 Recombinant antibody, PBS Only (Capture/Detector)

ESR2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240412F2

Cat no : 83447-3-PBS

Print datasheet

Synonyms

ESTRB, ERβ, ER-beta, Erb, ER BETA

验证数据展示

  • FC experiment of MCF-7 using 83447-3-RR (same clone as 83447-3-PBS)

    FC experiment of MCF-7 using 83447-3-RR (same clone as 83447-3-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Esr2 Recombinant Antibody (83447-3-RR, Clone:240412F2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83447-3-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83447-3-RR (same clone as 83447-3-PBS)

    IF Staining of HepG2 using 83447-3-RR (same clone as 83447-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ESR2 antibody (83447-3-RR, Clone: 240412F2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83447-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00441-1

    Cytometric bead array standard curve of MP00441-1

    Cytometric bead array standard curve of MP00441-1, ESR2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83447-2-PBS. Detection antibody: 83447-3-PBS. Standard: Ag5103. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP00441-2

    Cytometric bead array standard curve of MP00441-2

    Cytometric bead array standard curve of MP00441-2, ESR2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83447-3-PBS. Detection antibody: 83447-1-PBS. Standard: Ag5103. Range: 0.781-50 ng/mL.

  • Affinity and Kinetic Characterization of 83447-3-RR

    Affinity and Kinetic Characterization of 83447-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83447-3-RR against Human ESR2 were performed. The affinity constant is 0.22 nM.

产品信息

83447-3-PBS targets ESR2 as part of a matched antibody pair:

MP00441-1: 83447-2-PBS capture and 83447-3-PBS detection (validated in Cytometric bead array)

MP00441-2: 83447-3-PBS capture and 83447-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen ESR2 fusion protein Ag5103 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name estrogen receptor 2 (ER beta)
Synonyms ESTRB, ERβ, ER-beta, Erb, ER BETA
Calculated Molecular Weight 59 kDa
GenBank Accession NumberBC024181
Gene Symbol ESR2
Gene ID (NCBI) 2100
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92731
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C