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MIG6; ERRFI1 Recombinant antibody, PBS Only (Detector)

MIG6; ERRFI1 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240447C3

Cat no : 83475-2-PBS

Print datasheet

Synonyms

ERRFI1, 240447C3, MIG-6, MIG6, MIG 6

验证数据展示

  • WB analysis using 83475-2-RR (same clone as 83475-2-PBS)

    WB analysis using 83475-2-RR (same clone as 83475-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83475-2-RR (MIG6; ERRFI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83475-2-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83475-2-RR (same clone as 83475-2-PBS)

    FC experiment of HepG2 using 83475-2-RR (same clone as 83475-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug MIG6; ERRFI1 Recombinant antibody (83475-2-RR, Clone:240447C3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83475-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00464-3

    Cytometric bead array standard curve of MP00464-3

    Cytometric bead array standard curve of MP00464-3, MIG6; ERRFI1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83475-1-PBS. Detection antibody: 83475-2-PBS. Standard: Ag33341. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83475-2-RR

    Affinity and Kinetic Characterization of 83475-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83475-2-RR against Human MIG6; ERRFI1 were performed. The affinity constant is 2.04 nM.

产品信息

83475-2-PBS targets MIG6; ERRFI1 as part of a matched antibody pair:

MP00464-3: 83475-1-PBS capture and 83475-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen MIG6; ERRFI1 fusion protein Ag33341 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ERBB receptor feedback inhibitor 1
Synonyms ERRFI1, 240447C3, MIG-6, MIG6, MIG 6
Calculated Molecular Weight51kd
Observed Molecular Weight 51 kDa
GenBank Accession NumberNM_018948
Gene Symbol MIG6
Gene ID (NCBI) 54206
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UJM3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ERRFI1 (the product of mitogen-inducible gene 6, also known as MIG6) is an early response gene encoding a non-kinase scaffold adaptor protein induced by various stimuli such as hormones and stresses. ERRFI1 is considered a negative regulator of EGFR because it can directly bind to EGFR, inhibit the catalytic activity of EGFR, and mediate EGFR lysosomal degradation. ERRFI1 may play important roles in regulating stress response, maintaining homeostasis in tissues like joints or cardiac muscle, and functioning as a tumor suppressor.