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SLC35B4 Recombinant antibody

SLC35B4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240332F10

Cat no : 83527-3-RR

Print datasheet

Synonyms

YEA4, YEA, UDP-xylose and UDP-N-acetylglucosamine transporter, PSEC0055, Nucleotide sugar transporter SLC35B4

验证数据展示

  • WB analysis of DU 145 using 83527-3-RR

    WB analysis of DU 145 using 83527-3-RR

    DU 145 cells were subjected to SDS PAGE followed by western blot with 83527-3-RR (SLC35B4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83527-3-RR

    FC experiment of HepG2 using 83527-3-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Slc35b4 Recombinant Antibody (83527-3-RR, Clone:240332F10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83527-3-RR

    Affinity and Kinetic Characterization of 83527-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83527-3-RR against Human SLC35B4 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inDU 145 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83527-3-RR targets SLC35B4 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen SLC35B4 fusion protein Ag22669 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name solute carrier family 35, member B4
Synonyms YEA4, YEA, UDP-xylose and UDP-N-acetylglucosamine transporter, PSEC0055, Nucleotide sugar transporter SLC35B4
Observed Molecular Weight35-37 kDa
GenBank Accession NumberBC008413
Gene Symbol SLC35B4
Gene ID (NCBI) 84912
RRIDAB_3671154
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ969S0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC35B4 (solute carrier family 35 member B4), also known as YEA. It is expected to be located in the endoplasmic reticulum membrane, which is ubiquitinated in placenta and brain. SLC35B4 is a glycosyltransferase, which transports nucleotide sugars from the cytoplasm where they are synthesized, to the Golgi apparatus where they are utilized in the synthesis of glycoproteins, glycolipids, and proteoglycans (PMID: 15911612). Belongs to the nucleotide-sugar transporter family. SLC35B subfamily. The molecular weight of SLC35B4 is 37 kDa.

实验方案

Product Specific Protocols
WB protocol for SLC35B4 antibody 83527-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols