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验证数据展示

  • IHC staining of human skin cancer using 83577-1-RR (same clone as 83577-1-PBS)

    IHC staining of human skin cancer using 83577-1-RR (same clone as 83577-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 83577-1-RR (GAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83577-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83577-1-RR (same clone as 83577-1-PBS)

    IF Staining of HepG2 using 83577-1-RR (same clone as 83577-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GAR1 antibody (83577-1-RR, Clone: 240537C11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83577-1-PBS in a different storage buffer formulation.

  • FC experiment of K562 using 83577-1-RR (same clone as 83577-1-PBS)

    FC experiment of K562 using 83577-1-RR (same clone as 83577-1-PBS)

    1x10^6 K562 were intracellularly stained with 0.25 ug GAR1 Recombinant antibody (83577-1-RR, Clone:240537C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83577-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00568-1

    Cytometric bead array standard curve of MP00568-1

    Cytometric bead array standard curve of MP00568-1, GAR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83577-2-PBS. Detection antibody: 83577-1-PBS. Standard: Ag2282. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP00568-2

    Cytometric bead array standard curve of MP00568-2

    Cytometric bead array standard curve of MP00568-2, GAR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83577-3-PBS. Detection antibody: 83577-1-PBS. Standard: Ag2282. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 83577-1-RR

    Affinity and Kinetic Characterization of 83577-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83577-1-RR against Human GAR1 were performed. The affinity constant is below 1 pM.

GAR1 Recombinant antibody, PBS Only (Detector)

GAR1 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 83577-1-PBS

产品说明书

CloneNo. 240537C11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

snoRNP protein GAR1, NOLA1, 240537C11

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83577-1-PBS targets GAR1 as part of a matched antibody pair:

MP00568-1: 83577-2-PBS capture and 83577-1-PBS detection (validated in Cytometric bead array)

MP00568-2: 83577-3-PBS capture and 83577-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 GAR1 fusion protein Ag2282 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GAR1 ribonucleoprotein homolog (yeast)
别名 snoRNP protein GAR1, NOLA1, 240537C11
计算分子量 217 aa, 22 kDa
GenBank蛋白编号BC003413
基因名称 GAR1
Gene ID (NCBI) 54433
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NY12
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GAR1, other named NOLA1, is a subunit of H/ACA and telomerase. Thought is not required for H/ACA protein assembly, GAR1 is necessary for ribosome biogenesis and telomere. H/ACA involves in class specify the sites of uridine-to-pseudouridine. It contains a core domain that flanked by glycine- and arginine-rich(GAR) domains. GAR1 also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transciptase(TERT) holoenzyme.