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CCDC94 Recombinant antibody, PBS Only (Capture)

CCDC94 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240517A1

Cat no : 83580-1-PBS

Print datasheet

Synonyms

YJU2, Coiled-coil domain-containing protein 94, 240517A1

验证数据展示

  • WB analysis using 83580-1-RR (same clone as 83580-1-PBS)

    WB analysis using 83580-1-RR (same clone as 83580-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83580-1-RR (CCDC94 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83580-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 83580-1-RR (same clone as 83580-1-PBS)

    IF Staining of A549 using 83580-1-RR (same clone as 83580-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CCDC94 antibody (83580-1-RR, Clone: 240517A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83580-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 83580-1-RR (same clone as 83580-1-PBS)

    IF Staining of A549 using 83580-1-RR (same clone as 83580-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CCDC94 antibody (83580-1-RR, Clone: 240517A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83580-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 83580-1-RR (same clone as 83580-1-PBS)

    FC experiment of A431 using 83580-1-RR (same clone as 83580-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug CCDC94 Recombinant antibody (83580-1-RR, Clone:240517A1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83580-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00553-2

    Cytometric bead array standard curve of MP00553-2

    Cytometric bead array standard curve of MP00553-2, CCDC94 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83580-1-PBS. Detection antibody: 83580-2-PBS. Standard: Ag13764. Range: 0.78-100 ng/mL.

  • Cytometric bead array standard curve of MP00553-3

    Cytometric bead array standard curve of MP00553-3

    Cytometric bead array standard curve of MP00553-3, CCDC94 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83580-1-PBS. Detection antibody: 83580-3-PBS. Standard: Ag13764. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83580-1-RR

    Affinity and Kinetic Characterization of 83580-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83580-1-RR against Human CCDC94 were performed. The affinity constant is 1.08 nM.

产品信息

83580-1-PBS targets CCDC94 as part of a matched antibody pair:

MP00553-2: 83580-1-PBS capture and 83580-2-PBS detection (validated in Cytometric bead array)

MP00553-3: 83580-1-PBS capture and 83580-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen CCDC94 fusion protein Ag13764 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name coiled-coil domain containing 94
Synonyms YJU2, Coiled-coil domain-containing protein 94, 240517A1
Calculated Molecular Weight 323 aa, 37 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC000561
Gene Symbol CCDC94
Gene ID (NCBI) 55702
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BW85
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCDC94, also known as YJU2, is a splicing factor, predicted to be involved in RNA splicing and negative regulation of DNA damage response, signal transduction by p53 class mediator. Part of U2-type catalytic step 1 spliceosome. And that it is required for pre-mRNA splicing both in vivo and in vitro. Yju2 acts in concert with an unidentified heat-resistant factor(s) in an ATP-independent manner to promote the first catalytic reaction of pre-mRNA splicing after Prp2-mediated structural rearrangement of the spliceosome. (PMID: 17515604)