• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83591-2-RR

    WB analysis using 83591-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83591-2-RR (Drebrin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 83591-2-RR

    IHC staining of human stomach cancer using 83591-2-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83591-2-RR (Drebrin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

    Ready-to-use IHC kits available with DBN1/Drebrin antibody included
  • FC experiment of SH-SY5Y using 83591-2-RR

    FC experiment of SH-SY5Y using 83591-2-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Drebrin Recombinant antibody (83591-2-RR, Clone:240599G12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83591-2-RR

    Affinity and Kinetic Characterization of 83591-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83591-2-RR against Human DHRS3 were performed. The affinity constant is 0.109 nM.

Drebrin Recombinant antibody

Drebrin Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83591-2-RR

产品说明书

CloneNo. 240599G12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), ELISA

别名

DBN1, drebrin 1, Developmentally-regulated brain protein, DBN 1, 240599G12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, Neuro-2a cells, SH-SY5Y cells, PC-12 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy DBN1/Drebrin Ready-To-Use IHC Kit. DBN1/Drebrin primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83591-2-RR targets Drebrin in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Drebrin fusion protein Ag0371 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 drebrin 1
别名 DBN1, drebrin 1, Developmentally-regulated brain protein, DBN 1, 240599G12
计算分子量 71 kDa
观测分子量 110-130 kDa
GenBank蛋白编号BC000283
基因名称 Drebrin
Gene ID (NCBI) 1627
ENSEMBL Gene IDENSG00000113758
RRIDAB_3671206
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16643
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.

实验方案

Product Specific Protocols
WB protocol for Drebrin antibody 83591-2-RRDownload protocol
IHC protocol for Drebrin antibody 83591-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols