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验证数据展示

  • WB analysis using 83591-2-RR (same clone as 83591-2-PBS)

    WB analysis using 83591-2-RR (same clone as 83591-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83591-2-RR (Drebrin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83591-2-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 83591-2-RR (same clone as 83591-2-PBS)

    IHC staining of human stomach cancer using 83591-2-RR (same clone as 83591-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83591-2-RR (Drebrin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83591-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DBN1/Drebrin antibody included
  • FC experiment of SH-SY5Y using 83591-2-RR (same clone as 83591-2-PBS)

    FC experiment of SH-SY5Y using 83591-2-RR (same clone as 83591-2-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Drebrin Recombinant antibody (83591-2-RR, Clone:240599G12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83591-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00585-2

    Cytometric bead array standard curve of MP00585-2

    Cytometric bead array standard curve of MP00585-2, Drebrin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83591-2-PBS. Detection antibody: 83591-1-PBS. Standard: Ag0371. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83591-2-RR

    Affinity and Kinetic Characterization of 83591-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83591-2-RR against Human DHRS3 were performed. The affinity constant is 0.109 nM.

Drebrin Recombinant antibody, PBS Only (Capture)

Drebrin Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83591-2-PBS

产品说明书

CloneNo. 240599G12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

DBN1, drebrin 1, Developmentally-regulated brain protein, DBN 1, 240599G12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy DBN1/Drebrin Ready-To-Use IHC Kit. DBN1/Drebrin primary antibody included.

产品信息

83591-2-PBS targets Drebrin as part of a matched antibody pair:

MP00585-2: 83591-2-PBS capture and 83591-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Drebrin fusion protein Ag0371 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 drebrin 1
别名 DBN1, drebrin 1, Developmentally-regulated brain protein, DBN 1, 240599G12
计算分子量 71 kDa
观测分子量 110-130 kDa
GenBank蛋白编号BC000283
基因名称 Drebrin
Gene ID (NCBI) 1627
ENSEMBL Gene IDENSG00000113758
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16643
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.