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BARHL2 Recombinant antibody

BARHL2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240558G8

Cat no : 83596-4-RR

Print datasheet

Synonyms

BarH-like 2 homeobox protein, BarH like 2 homeobox protein, 240558G8

验证数据展示

  • WB analysis using 83596-4-RR

    WB analysis using 83596-4-RR

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 83596-4-RR (BARHL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 83596-4-RR

    IF Staining of HepG2 using 83596-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BARHL2 antibody (83596-4-RR, Clone: 240558G8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 83596-4-RR

    IF Staining of HepG2 using 83596-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BARHL2 antibody (83596-4-RR, Clone: 240558G8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83596-4-RR

    Affinity and Kinetic Characterization of 83596-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83596-4-RR against Human BARHL2 were performed. The affinity constant is 0.478 nM.

经过测试的应用

Positive WB detected inmouse cerebellum tissue, rat cerebellum tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83596-4-RR is BARHL2 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen BARHL2 fusion protein Ag21156 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name BarH-like homeobox 2
Synonyms BarH-like 2 homeobox protein, BarH like 2 homeobox protein, 240558G8
Calculated Molecular Weight 387 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC126439
Gene Symbol BARHL2
Gene ID (NCBI) 343472
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NY43
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BARHL2 also named as BarH like homeobox 2 is 279 amino acid protein, which contains 1 homeobox DNA-binding domain. BARHL2 localizes in nucleus and belongs to BAR homeobox family. BARHL2 as a transcription factor binds optimally to the DNA consensus sequence. Barx2 may differentially control the expression of L1 and other target genes during embryonic development. Barx2 is expressed in several smooth muscle-containing tissues, as well as skeletal muscle, brain, tongue and esophagus.

实验方案

Product Specific Protocols
WB protocol for BARHL2 antibody 83596-4-RRDownload protocol
IF protocol for BARHL2 antibody 83596-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols