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验证数据展示

  • IHC staining of mouse skeletal muscle using 83609-6-RR

    IHC staining of mouse skeletal muscle using 83609-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83609-6-RR (Dystrophin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Dystrophin/DMD antibody included
  • IF Staining of rat brain using 83609-6-RR

    IF Staining of rat brain using 83609-6-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using Dystrophin antibody (83609-6-RR, Clone: 240567C4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat brain using 83609-6-RR

    IF Staining of rat brain using 83609-6-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using Dystrophin antibody (83609-6-RR, Clone: 240567C4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HepG2 using 83609-6-RR

    FC experiment of HepG2 using 83609-6-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug DMD Recombinant antibody (83609-6-RR, Clone:240567C4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83609-6-RR

    Affinity and Kinetic Characterization of 83609-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83609-6-RR against Human Dystrophin were performed. The affinity constant is below 1 pM.

Dystrophin Recombinant antibody

Dystrophin Uni-rAbTM Recombinant Antibody for IHC, IF-P, FC (Intra), ELISA
Cat No. 83609-6-RR

产品说明书

CloneNo. 240567C4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF-P, FC (Intra), ELISA

别名

DMD, BMD, 240567C4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy Dystrophin/DMD Ready-To-Use IHC Kit. Dystrophin/DMD primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83609-6-RR targets Dystrophin in IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Dystrophin fusion protein Ag3408 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 dystrophin
别名 DMD, BMD, 240567C4
计算分子量 3685 aa, 427 kDa
GenBank蛋白编号BC028720
基因名称 Dystrophin
Gene ID (NCBI) 1756
RRIDAB_3671224
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP11532
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscle weakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of the dystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin is located on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma and extracellular matrix. Multiple isoforms of dystrophin exist due to the alternative splicing, with a wide range of MW (69-72, 110-143, 271, 426 kDa). Most tissues contain transcripts of several isoforms.

实验方案

Product Specific Protocols
IHC protocol for Dystrophin antibody 83609-6-RRDownload protocol
IF protocol for Dystrophin antibody 83609-6-RRDownload protocol
FC protocol for Dystrophin antibody 83609-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols