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验证数据展示

  • IHC staining of human ovary cancer using 83636-6-RR

    IHC staining of human ovary cancer using 83636-6-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83636-6-RR (ATP6V1E1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATP6V1E1 antibody included
  • WB analysis using 83636-6-RR

    WB analysis using 83636-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83636-6-RR (ATP6V1E1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83636-6-RR

    Affinity and Kinetic Characterization of 83636-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83636-6-RR against Human ATP6V1E1 were performed. The affinity constant is 5.43 nM.

ATP6V1E1 Recombinant antibody

ATP6V1E1 Recombinant Antibody for WB, IHC, ELISA
Cat No. 83636-6-RR

产品说明书

CloneNo. 240584G12

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, ELISA

别名

V-ATPase subunit E 1, V-ATPase 31 kDa subunit, P31, ATP6E2, ATP6E

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, U2OS cells, rat brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ATP6V1E1 Ready-To-Use IHC Kit. ATP6V1E1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83636-6-RR targets ATP6V1E1 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, rat
免疫原 ATP6V1E1 fusion protein Ag7399 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1
别名 V-ATPase subunit E 1, V-ATPase 31 kDa subunit, P31, ATP6E2, ATP6E
计算分子量 26 kDa
观测分子量31 kDa
GenBank蛋白编号BC004443
基因名称 ATP6V1E1
Gene ID (NCBI) 529
RRIDAB_3671248
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP36543
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP6V1E1, also named as ATP6E, ATP6E2 and p31, belongs to the V-ATPase E subunit family. It is a subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. V-ATPase and the lack of mycobacterial phagosome acidification are directly attributed to the Mtb protein tyrosine phosphatase PtpA.

实验方案

Product Specific Protocols
WB protocol for ATP6V1E1 antibody 83636-6-RRDownload protocol
IHC protocol for ATP6V1E1 antibody 83636-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols