• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ATP6V1E1 Recombinant antibody, PBS Only

ATP6V1E1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240584G12

Cat no : 83636-6-PBS

Print datasheet

Synonyms

V-ATPase subunit E 1, V-ATPase 31 kDa subunit, P31, ATP6E2, ATP6E

验证数据展示

  • IHC staining of human ovary cancer using 83636-6-RR (same clone as 83636-6-PBS)

    IHC staining of human ovary cancer using 83636-6-RR (same clone as 83636-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83636-6-RR (ATP6V1E1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83636-6-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATP6V1E1 antibody included
  • WB analysis using 83636-6-RR (same clone as 83636-6-PBS)

    WB analysis using 83636-6-RR (same clone as 83636-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83636-6-RR (ATP6V1E1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83636-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83636-6-RR

    Affinity and Kinetic Characterization of 83636-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83636-6-RR against Human ATP6V1E1 were performed. The affinity constant is 5.43 nM.

Planning an IHC experiment? We recommend our IHCeasy ATP6V1E1 Ready-To-Use IHC Kit. ATP6V1E1 primary antibody included.

产品信息

83636-6-PBS targets ATP6V1E1 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, rat
Immunogen ATP6V1E1 fusion protein Ag7399 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1
Synonyms V-ATPase subunit E 1, V-ATPase 31 kDa subunit, P31, ATP6E2, ATP6E
Calculated Molecular Weight 26 kDa
Observed Molecular Weight31 kDa
GenBank Accession NumberBC004443
Gene Symbol ATP6V1E1
Gene ID (NCBI) 529
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP36543
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP6V1E1, also named as ATP6E, ATP6E2 and p31, belongs to the V-ATPase E subunit family. It is a subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. V-ATPase and the lack of mycobacterial phagosome acidification are directly attributed to the Mtb protein tyrosine phosphatase PtpA.