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验证数据展示

  • IHC staining of human ovary cancer using 83636-6-RR (same clone as 83636-6-PBS)

    IHC staining of human ovary cancer using 83636-6-RR (same clone as 83636-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83636-6-RR (ATP6V1E1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83636-6-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATP6V1E1 antibody included
  • WB analysis using 83636-6-RR (same clone as 83636-6-PBS)

    WB analysis using 83636-6-RR (same clone as 83636-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83636-6-RR (ATP6V1E1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83636-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83636-6-RR

    Affinity and Kinetic Characterization of 83636-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83636-6-RR against Human ATP6V1E1 were performed. The affinity constant is 5.43 nM.

ATP6V1E1 Recombinant antibody, PBS Only

ATP6V1E1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83636-6-PBS

产品说明书

CloneNo. 240584G12

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, ELISA

别名

V-ATPase subunit E 1, V-ATPase 31 kDa subunit, P31, ATP6E2, ATP6E

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ATP6V1E1 Ready-To-Use IHC Kit. ATP6V1E1 primary antibody included.

产品信息

83636-6-PBS targets ATP6V1E1 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, rat
免疫原 ATP6V1E1 fusion protein Ag7399 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1
别名 V-ATPase subunit E 1, V-ATPase 31 kDa subunit, P31, ATP6E2, ATP6E
计算分子量 26 kDa
观测分子量31 kDa
GenBank蛋白编号BC004443
基因名称 ATP6V1E1
Gene ID (NCBI) 529
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP36543
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP6V1E1, also named as ATP6E, ATP6E2 and p31, belongs to the V-ATPase E subunit family. It is a subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. V-ATPase and the lack of mycobacterial phagosome acidification are directly attributed to the Mtb protein tyrosine phosphatase PtpA.