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验证数据展示

  • IHC staining of human ovary cancer using 83636-6-RR

    IHC staining of human ovary cancer using 83636-6-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83636-6-RR (ATP6V1E1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATP6V1E1 antibody included
  • WB analysis using 83636-6-RR

    WB analysis using 83636-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83636-6-RR (ATP6V1E1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83636-6-RR

    Affinity and Kinetic Characterization of 83636-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83636-6-RR against Human ATP6V1E1 were performed. The affinity constant is 5.43 nM.

ATP6V1E1 Recombinant antibody

ATP6V1E1 Recombinant Antibody for WB, IHC, ELISA
Cat No. 83636-6-RR
CloneNo. 240584G12

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Synonyms

V-ATPase subunit E 1, V-ATPase 31 kDa subunit, P31, ATP6E2, ATP6E

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, U2OS cells, rat brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ATP6V1E1 Ready-To-Use IHC Kit. ATP6V1E1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83636-6-RR targets ATP6V1E1 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, rat
Immunogen ATP6V1E1 fusion protein Ag7399 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1
Synonyms V-ATPase subunit E 1, V-ATPase 31 kDa subunit, P31, ATP6E2, ATP6E
Calculated Molecular Weight 26 kDa
Observed Molecular Weight31 kDa
GenBank Accession NumberBC004443
Gene Symbol ATP6V1E1
Gene ID (NCBI) 529
RRIDAB_3671248
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP36543
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP6V1E1, also named as ATP6E, ATP6E2 and p31, belongs to the V-ATPase E subunit family. It is a subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. V-ATPase and the lack of mycobacterial phagosome acidification are directly attributed to the Mtb protein tyrosine phosphatase PtpA.

实验方案

Product Specific Protocols
WB protocol for ATP6V1E1 antibody 83636-6-RRDownload protocol
IHC protocol for ATP6V1E1 antibody 83636-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols