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验证数据展示

  • WB analysis using 83638-3-RR (same clone as 83638-3-PBS)

    WB analysis using 83638-3-RR (same clone as 83638-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83638-3-RR (RCN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83638-3-PBS in a different storage buffer formulation.

  • WB analysis using 83638-3-RR (same clone as 83638-3-PBS)

    WB analysis using 83638-3-RR (same clone as 83638-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83638-3-RR (RCN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83638-3-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 83638-3-RR (same clone as 83638-3-PBS)

    IF Staining of U2OS using 83638-3-RR (same clone as 83638-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RCN1 antibody (83638-3-RR, Clone: 240473E9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83638-3-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83638-3-RR (same clone as 83638-3-PBS)

    FC experiment of HeLa using 83638-3-RR (same clone as 83638-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RCN1 Recombinant antibody (83638-3-RR, Clone:240473E9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83638-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00637-3

    Cytometric bead array standard curve of MP00637-3

    Cytometric bead array standard curve of MP00637-3, RCN1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83638-4-PBS. Detection antibody: 83638-3-PBS. Standard: Ag13256. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83638-3-PBS

    Affinity and Kinetic Characterization of 83638-3-PBS

    Biolayer interferometry (BLl) kinetic assay of 83638-3-PBS against Human RCN1 was performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 83638-3-RR

    Affinity and Kinetic Characterization of 83638-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83638-3-RR against Human RCN1 were performed. The affinity constant is below 1 pM.

RCN1 Recombinant antibody, PBS Only (Detector)

RCN1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83638-3-PBS

产品说明书

CloneNo. 240473E9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

Reticulocalbin 1, Reticulocalbin-1, RCN, RCAL, PIG20

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83638-3-PBS targets RCN1 as part of a matched antibody pair:

MP00637-3: 83638-4-PBS capture and 83638-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 RCN1 fusion protein Ag13256 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 reticulocalbin 1, EF-hand calcium binding domain
别名 Reticulocalbin 1, Reticulocalbin-1, RCN, RCAL, PIG20
计算分子量 39 kDa
观测分子量 44-46 kDa
GenBank蛋白编号BC010120
基因名称 RCN1
Gene ID (NCBI) 5954
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15293
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Reticulocalbin-1 (RCN1) belongs to the CREC family. Reticulocalbin‐1, an endoplasmic reticulum protein, plays a crucial role in calcium homeostasis and inhibits ER stress‐induced apoptosis (PMID: 28319095). Its main functions include maintaining intracellular homeostasis and regulating cell proliferation and apoptosis, and it plays an important role in the development of various tumours (PMID: 38057406). RCN1 is expressed aberrantly and at a high level in various tumors, including acute myeloid leukemia (AML) (PMID: 37746742). In multiple cancers, such as glioblastoma, non‐small cell lung cancer, renal cell carcinoma, hepatocellular carcinoma, and oral squamous cell carcinoma, the overexpression of RCN1 has been observed indicating its involvement in tumorigenesis and invasion (PMID: 30172915, PMID: 34722631).