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Species Reactivity
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验证数据展示

  • WB analysis using 83648-1-RR (same clone as 83648-1-PBS)

    WB analysis using 83648-1-RR (same clone as 83648-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83648-1-RR (MNT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83648-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00617-1

    Cytometric bead array standard curve of MP00617-1

    Cytometric bead array standard curve of MP00617-1, MNT Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83648-1-PBS. Detection antibody: 83648-3-PBS. Standard: Ag34529. Range: 0.625-80 ng/mL.

  • Sandwich ELISA standard curve of MP00617-1

    Sandwich ELISA standard curve of MP00617-1

    Sandwich ELISA standard curve of MP00617-1, Human MNT Recombinant Matched Antibody Pair - PBS only. 83648-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34529. 83648-3-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP00617-1

    Sample test of MP00617-1

    The mean MNT concentration was determined to be 19.62 ng/mL in HEK-293T cell extract based on a 5.50 mg/mL extract load and 17.06 ng/mL in HeLa cell extract based on a 3.60 mg/mL extract load.

  • FC experiment of A431 using 83648-1-RR (same clone as 83648-1-PBS)

    FC experiment of A431 using 83648-1-RR (same clone as 83648-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MNT Recombinant antibody (83648-1-RR, Clone:240550A10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83648-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83648-1-RR (same clone as 83648-1-PBS)

    IF Staining of HeLa using 83648-1-RR (same clone as 83648-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MNT antibody (83648-1-RR, Clone: 240550A10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83648-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00617-2

    Cytometric bead array standard curve of MP00617-2

    Cytometric bead array standard curve of MP00617-2, MNT Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83648-1-PBS. Detection antibody: 83648-4-PBS. Standard: Ag34529. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00617-3

    Cytometric bead array standard curve of MP00617-3

    Cytometric bead array standard curve of MP00617-3, MNT Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83648-1-PBS. Detection antibody: 83648-2-PBS. Standard: Ag34529. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83648-1-RR

    Affinity and Kinetic Characterization of 83648-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83648-1-RR against Human MNT were performed. The affinity constant is below 1 pM.

MNT Recombinant antibody, PBS Only (Capture)

MNT Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83648-1-PBS

产品说明书

CloneNo. 240550A10

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

Max binding protein MNT, MAX binding protein, MAD6, Class D basic helix-loop-helix protein 3, bHLHd3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MNT Ready-To-Use IHC Kit. MNT primary antibody included.

产品信息

83648-1-PBS targets MNT as part of a matched antibody pair:

MP00617-1: 83648-1-PBS capture and 83648-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00617-2: 83648-1-PBS capture and 83648-4-PBS detection (validated in Cytometric bead array)

MP00617-3: 83648-1-PBS capture and 83648-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, rat
免疫原 MNT fusion protein Ag34529 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 MAX binding protein
别名 Max binding protein MNT, MAX binding protein, MAD6, Class D basic helix-loop-helix protein 3, bHLHd3
计算分子量 582 aa, 62 kDa
观测分子量 70 kDa
GenBank蛋白编号BC117563
基因名称 MNT
Gene ID (NCBI) 4335
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99583
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mnt (Max binding protein), also known as MAD6, ROX, bHLHd3 (class D basic helix-loop-helix protein 3) or MXD6, is a 582 amino acid nuclear protein that forms a complex with Max (Myc-associated factor X) to repress transcription. Mnt contains one basic helix-loop-helix (bHLH) domain and is encoded by a gene that maps to human chromosome 17p13.3. Chromosome 17 comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes.