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验证数据展示

  • WB analysis using 83683-6-RR

    WB analysis using 83683-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83683-6-RR (Beta-2-Microglobulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of A431 using 83683-6-RR

    IP experiment of A431 using 83683-6-RR

    IP result of anti-Beta-2-Microglobulin (IP:83683-6-RR, 4ug; Detection:83683-6-RR 1:3000) with A431 cells lysate 2180 ug.

  • IHC staining of human tonsillitis using 83683-6-RR

    IHC staining of human tonsillitis using 83683-6-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83683-6-RR (Beta-2-Microglobulin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Beta-2-Microglobulin antibody included
  • IHC staining of human tonsillitis using 83683-6-RR

    IHC staining of human tonsillitis using 83683-6-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83683-6-RR (Beta-2-Microglobulin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Beta-2-Microglobulin antibody included
  • Affinity and Kinetic Characterization of 83683-6-RR

    Affinity and Kinetic Characterization of 83683-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83683-6-RR against Human Beta-2-Microglobulin were performed. The affinity constant is 1.18 nM.

Beta-2-Microglobulin Recombinant antibody

Beta-2-Microglobulin Uni-rAbTM Recombinant Antibody for WB, IHC, IP, ELISA
Cat No. 83683-6-RR

产品说明书

CloneNo. 240660A12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA

别名

B2M, 240660A12, beta 2 microglobulin, beta 2-Microglobulin, Beta-2-microglobulin form pI 5.3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, Raji cells, U-937 cells, Jurkat cells
Positive IP detected inA431 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Beta-2-Microglobulin Ready-To-Use IHC Kit. Beta-2-Microglobulin primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83683-6-RR targets Beta-2-Microglobulin in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human
免疫原 Beta-2-Microglobulin fusion protein Ag4433 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 beta-2-microglobulin
别名 B2M, 240660A12, beta 2 microglobulin, beta 2-Microglobulin, Beta-2-microglobulin form pI 5.3
计算分子量 119 aa, 14 kDa
观测分子量 12 kDa
GenBank蛋白编号BC032589
基因名称 B2M
Gene ID (NCBI) 567
ENSEMBL Gene IDENSG00000166710
RRIDAB_3671284
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP61769
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions due to shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.

实验方案

Product Specific Protocols
WB protocol for Beta-2-Microglobulin antibody 83683-6-RRDownload protocol
IHC protocol for Beta-2-Microglobulin antibody 83683-6-RRDownload protocol
IP protocol for Beta-2-Microglobulin antibody 83683-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols