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Rat SCF Recombinant antibody, PBS Only (Detector)

SCF Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240787H1

Cat no : 83707-2-PBS

Print datasheet

Synonyms

Kitlg, Mgf, Kitl, KIT ligand, 240787H1

验证数据展示

  • WB analysis using 83707-2-RR (same clone as 83707-2-PBS)

    WB analysis using 83707-2-RR (same clone as 83707-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83707-2-RR (SCF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83707-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00664-1

    Sandwich ELISA standard curve of MP00664-1

    Sandwich ELISA standard curve of MP00664-1, Rat SCF Recombinant Matched Antibody Pair - PBS only. 83707-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1092. 83707-2-PBS was HRP conjugated as the detection antibody. Range: 46.9-3000 pg/mL

  • Sample test of MP00664-1

    Sample test of MP00664-1

    Serum of eight rats was measured. The rat SCF concentration of detected samples was determined to be 191.0 pg/mL with a range of 14.0 - 481.6 pg/mL.

  • Cytometric bead array standard curve of MP00664-1

    Cytometric bead array standard curve of MP00664-1

    Cytometric bead array standard curve of MP00664-1, RAT SCF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83707-1-PBS. Detection antibody: 83707-2-PBS. Standard: Eg1092. Range: 0.313-20 ng/mL

  • Affinity and Kinetic Characterization of 83707-2-RR

    Affinity and Kinetic Characterization of 83707-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83707-2-RR against Rat SCF were performed. The affinity constant is 0.624 nM.

产品信息

83707-2-PBS targets SCF as part of a matched antibody pair:

MP00664-1: 83707-1-PBS capture and 83707-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human, mouse, rat
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name KIT ligand
Synonyms Kitlg, Mgf, Kitl, KIT ligand, 240787H1
Calculated Molecular Weight31kDa
Observed Molecular Weight 28 kDa, 45 kDa
GenBank Accession NumberNM_021843.4
Gene Symbol Kitlg
Gene ID (NCBI) 60427
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21581-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Stem cell factor (SCF) also known as mast cell growth factor, steel factor, and kit ligand, is a hematopoietic growth factor. SCF is a critical protein with key roles in the cell such as hematopoiesis, gametogenesis and melanogenesis. SCF activates multiple signal pathways by binding with c-kit receptor. SCF and its receptor, c-kit ligand (KL), are up-regulated in particular human malignancies including gastrointestinal stromal tumor (GISTs), breast cancer, hematopoietic cell, myeloid leukaemia, and glioma. The SCF/c-KIT system also plays a relevant role in cell fate decision, and its activity is widely known to control cell proliferation, differentiation, survival and apoptosis. SCF is a 35 to 45 kDa monomeric glycoprotein.