• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83841-2-RR (same clone as 83841-2-PBS)

    WB analysis using 83841-2-RR (same clone as 83841-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83841-2-RR (SMAD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83841-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00791-1

    Sandwich ELISA standard curve of MP00791-1

    Sandwich ELISA standard curve of MP00791-1, Human SMAD2 Recombinant Matched Antibody Pair - PBS only. 83841-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3237. 83841-3-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

  • Sample test of MP00791-1

    Sample test of MP00791-1

    HEK-293T, HeLa, HepG2 and MCF-7 cell lysates were measured. The human SMAD2 concentration of detected samples was determined to be 43.85 ng/mL in HEK-293T cell lysate (based on a 5.5 mg/mL extract load). The human SMAD2 concentration of detected samples was determined to be 33.98 ng/mL in HeLa cell lysate (based on a 7 mg/mL extract load). The human SMAD2 concentration of detected samples was determined to be 24.50 ng/mL in HepG2 cell lysate (based on a 4.0 mg/mL extract load). The human SMAD2 concentration of detected samples was determined to be 12.02 ng/mL in MCF-7 cell lysate (based on a 3.1 mg/mL extract load).

  • Sample test of MP00791-1

    Sample test of MP00791-1

    Serum of sixteen individual healthy human donors was measured. The human SMAD2 concentration of detected samples was determined to be 2.17 ng/mL with a range of 0.34 - 8.22 ng/mL.

  • Cytometric bead array standard curve of MP00791-1

    Cytometric bead array standard curve of MP00791-1

    Cytometric bead array standard curve of MP00791-1, SMAD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83841-2-PBS. Detection antibody: 83841-3-PBS. Standard: Ag3237. Range: 0.469-60 ng/mL.

  • Cytometric bead array standard curve of MP00791-3

    Cytometric bead array standard curve of MP00791-3

    Cytometric bead array standard curve of MP00791-3, SMAD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83841-2-PBS. Detection antibody: 83841-1-PBS. Standard: Ag3237. Range: 0.938-60 ng/mL.

SMAD2 Recombinant antibody, PBS Only (Capture)

SMAD2 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83841-2-PBS

产品说明书

CloneNo. 240950G3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

JV18 1, JV18, hSMAD2, hMAD-2, hMAD 2

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 
100 μg

¥5800/ 100 μg

浓度: 1 mg/ml

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy SMAD2 Ready-To-Use IHC Kit. SMAD2 primary antibody included.

产品信息

83841-2-PBS targets SMAD2 as part of a matched antibody pair:

MP00791-1: 83841-2-PBS capture and 83841-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00791-3: 83841-2-PBS capture and 83841-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 SMAD2 fusion protein Ag3237 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SMAD family member 2
别名 JV18 1, JV18, hSMAD2, hMAD-2, hMAD 2
计算分子量 467 aa, 52 kDa
观测分子量58 kDa
GenBank蛋白编号BC014840
基因名称 SMAD2
Gene ID (NCBI) 4087
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15796
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMAD2, also named as MADH2 and MADR2, belongs to the dwarfin/SMAD family, contains 1 MH1 (MAD homology 1) domain and 1 MH2 (MAD homology 2) domain. SMAD2 is a receptor-regulated SMAD(R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. This protein may act as a tumor suppressor in colorectal carcinoma. It is phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, It is phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases, and then able to interact with SMURF2, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, it is phosphorylated on Ser-240 by CaMK2. It is phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. In response to TGF-beta, it is ubiquitinated by NEDD4L, which promotes its degradation. In response to TGF-beta signaling, it is acetylated on Lys-19 by coactivators, which increases transcriptional activity. The molecular weight of unphosphorylated forms of Smad2 is 52 kDa and phosphorylated forms of Smad2 is 58 kDa. (PMID: 9006934)