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VWF Recombinant antibody

VWF Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240867D10

Cat no : 83854-2-RR

Print datasheet

Synonyms

VWF, VWFpp, VWD, von Willebrand antigen II, F8VWF, 240867D10

验证数据展示

  • WB analysis using 83854-2-RR

    WB analysis using 83854-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83854-2-RR (vwf antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83854-2-RR

    Affinity and Kinetic Characterization of 83854-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83854-2-RR against Human VWF were performed. The affinity constant is 0.965 nM.

经过测试的应用

Positive WB detected inhuman placenta tissue, rat spleen tissue
Planning an IHC experiment? We recommend our IHCeasy VWF Ready-To-Use IHC Kit. VWF primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83854-2-RR is VWF Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen VWF fusion protein Ag25578 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name von Willebrand factor
Synonyms VWF, VWFpp, VWD, von Willebrand antigen II, F8VWF, 240867D10
Observed Molecular Weight309-320 kDa
Gene Symbol VWF
Gene ID (NCBI) 7450
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04275
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Von Willebrand factor (VWF) is a large multimeric glycoprotein found in blood plasma involved in hemostasis following vascular injury. Due to the multimeric nature of VWF, it can range in size from 500 to 20,000 kDa due to the differences in the number of subunits comprising the protein. Each subunit is approximately 250 kDa (PMID: 9759493). The biosynthesis of VWF in vivo is limited to endothelial cells (PMID: 4209883) and megakaryocytes (PMID: 2413071). VWF synthesized in endothelial cells is either released directly into the plasma via 27186a secretory pathway, or tubulized and stored in organelles unique to this cell type called Weibel-Palade bodies (PMID: 16459301). Whereas VWF synthesized in megakaryocytes is stored in the alpha granules of platelets (PMID: 2046403). The primary function of VWF is as an adhesive plasma glycoprotein, particularly factor VIII; an essential blood-clotting protein (PMID: 6982084). VWF is also important in platelet adhesion to wound sites by binding specifically to type I and type III collagen (PMID: 11098050), with larger VWF multimers being most effective (PMID: 24448155).

实验方案

Product Specific Protocols
WB protocol for VWF antibody 83854-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols