IHCeasy VWF Ready-To-Use IHC Kit
VWF Ready-to-use reagent kit for IHC.
Reactivity
Human
Sample Type
FFPE tissue
Cat No : KHC0209
Synonyms
F8VWF, von Willebrand antigen II, von Willebrand factor, VWD, VWF
Validation Data Gallery
Product Information
KHC0209 is a ready-to-use IHC kit for staining of VWF (von Willebrand factor). The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy VWF Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Mouse Monoclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Mouse |
| Reactivity | Human |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
Von Willebrand factor (VWF) is a large multimeric glycoprotein found in blood plasma involved in hemostasis following vascular injury. The biosynthesis of VWF in vivo is limited to endothelial cells and megakaryocytes. VWF synthesized in endothelial cells is either released directly into the plasma via 27186a secretory pathway, or tubulized and stored in organelles unique to this cell type called Weibel-Palade bodies. Whereas VWF synthesized in megakaryocytes is stored in the alpha granules of platelets. The primary function of VWF is as an adhesive plasma glycoprotein, particularly factor VIII; an essential blood-clotting protein. VWF is also important in platelet adhesion to wound sites by binding specifically to type I and type III collagen, with larger VWF multimers being most effective.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | F8VWF, von Willebrand antigen II, von Willebrand factor, VWD, VWF |