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YTHDC2 Recombinant antibody, PBS Only (Detector)

YTHDC2 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241123A9

Cat no : 83970-1-PBS

Print datasheet

Synonyms

YTH domain-containing protein 2, hYTHDC2, EC:3.6.4.13, 3'-5' RNA helicase YTHDC2, 241123A9

验证数据展示

  • WB analysis using 83970-1-RR (same clone as 83970-1-PBS)

    WB analysis using 83970-1-RR (same clone as 83970-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83970-1-RR (YTHDC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83970-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83970-1-RR (same clone as 83970-1-PBS)

    FC experiment of HepG2 using 83970-1-RR (same clone as 83970-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug YTHDC2 Recombinant antibody (83970-1-RR, Clone:241123A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83970-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00893-3

    Sandwich ELISA standard curve of MP00893-3

    Sandwich ELISA standard curve of MP00893-3, Human YTHDC2 Recombinant Matched Antibody Pair - PBS only. 83970-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag26690. 83970-1-PBS was HRP conjugated as the detection antibody. Range: 0.625-20 ng/mL

  • Sample test of MP00893-3

    Sample test of MP00893-3

    The mean YTHDC2 concentration was determined to be 4.89 ng/mL in HEK-293T cell extract based on a 1.4 mg/mL extract load.

  • IHC staining of rat testis using 83970-1-RR (same clone as 83970-1-PBS)

    IHC staining of rat testis using 83970-1-RR (same clone as 83970-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 83970-1-RR (YTHDC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83970-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YTHDC2 antibody included
  • IHC staining of rat testis using 83970-1-RR (same clone as 83970-1-PBS)

    IHC staining of rat testis using 83970-1-RR (same clone as 83970-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 83970-1-RR (YTHDC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83970-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YTHDC2 antibody included
  • Cytometric bead array standard curve of MP00893-1

    Cytometric bead array standard curve of MP00893-1

    Cytometric bead array standard curve of MP00893-1, YTHDC2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83970-4-PBS. Detection antibody: 83970-1-PBS. Standard: Ag26690. Range: 0.156-20 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy YTHDC2 Ready-To-Use IHC Kit. YTHDC2 primary antibody included.

产品信息

83970-1-PBS targets YTHDC2 as part of a matched antibody pair:

MP00893-1: 83970-4-PBS capture and 83970-1-PBS detection (validated in Cytometric bead array)

MP00893-3: 83970-3-PBS capture and 83970-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human, mouse
Immunogen YTHDC2 fusion protein Ag26690 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name YTH domain containing 2
Synonyms YTH domain-containing protein 2, hYTHDC2, EC:3.6.4.13, 3'-5' RNA helicase YTHDC2, 241123A9
Observed Molecular Weight160 kDa
GenBank Accession NumberBC137285
Gene Symbol YTHDC2
Gene ID (NCBI) 64848
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H6S0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

YTHDC2, also named as Probable ATP-dependent RNA helicase YTHDC2, is a 1430 amino acid protein, which belongs to the DEAD box helicase family. YTHDC2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.