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验证数据展示

  • WB analysis using 84083-2-RR (same clone as 84083-2-PBS)

    WB analysis using 84083-2-RR (same clone as 84083-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84083-2-RR (VGLUT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84083-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 84083-2-RR (same clone as 84083-2-PBS)

    IF Staining of mouse brain using 84083-2-RR (same clone as 84083-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using VGLUT2 antibody (84083-2-RR, Clone: 241255B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84083-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00998-1

    Cytometric bead array standard curve of MP00998-1

    Cytometric bead array standard curve of MP00998-1, SLC17A6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84083-1-PBS. Detection antibody: 84083-2-PBS. Standard: Ag29565. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84083-2-RR

    Affinity and Kinetic Characterization of 84083-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84083-2-RR against Human VGLUT2 were performed. The affinity constant is 1.48 nM.

VGLUT2 Recombinant antibody, PBS Only (Detector)

VGLUT2 Uni-rAbTM Recombinant Antibody for WB, IF-Fro, Cytometric bead array, Indirect ELISA
Cat No. 84083-2-PBS

产品说明书

CloneNo. 241255B5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-Fro, Cytometric bead array, Indirect ELISA

别名

SLC17A6, 241255B5, Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, DNPI

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84083-2-PBS targets VGLUT2 as part of a matched antibody pair:

MP00998-1: 84083-1-PBS capture and 84083-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF-Fro, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 VGLUT2 fusion protein Ag29565 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
别名 SLC17A6, 241255B5, Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, DNPI
计算分子量 64 kDa
观测分子量60 kDa
GenBank蛋白编号NM_020346
基因名称 VGLUT2
Gene ID (NCBI) 57084
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9P2U8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VGLUT2, also known as SLC17A6, belongs to the major facilitator superfamily. VGLUT2 is a multifunctional transporter that transports phosphate at the plasma membrane and glutamate in synaptic vesicles (PMID:33440152, 11432869). VGLUT2 is involved in neurotransmitter loading into synaptic vesicles (PMID: 11698620). VGLUT2 is predominantly expressed in adult and fetal brain, with highest expression in the medulla, substantia nigra, subthalamic nucleus, and thalamus, and low levels in the cerebellum and hippocampus (PMID:10820226).