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验证数据展示

  • WB analysis using 84107-2-RR (same clone as 84107-2-PBS)

    WB analysis using 84107-2-RR (same clone as 84107-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84107-2-RR (PROS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84107-2-PBS in a different storage buffer formulation.

  • WB analysis of DU 145 using 84107-2-RR (same clone as 84107-2-PBS)

    WB analysis of DU 145 using 84107-2-RR (same clone as 84107-2-PBS)

    WB result of PROS1 antibody (84107-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PROS1 transfected DU 145 cells. This data was developed using the same antibody clone with 84107-2-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 84107-2-RR (same clone as 84107-2-PBS)

    FC experiment of A549 using 84107-2-RR (same clone as 84107-2-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug PROS1 Recombinant antibody (84107-2-RR, Clone:241241E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84107-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01044-1

    Sandwich ELISA standard curve of MP01044-1

    Sandwich ELISA standard curve of MP01044-1, Human PROS1 Recombinant Matched Antibody Pair - PBS only. 84107-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10539. 84107-1-PBS was HRP conjugated as the detection antibody. Range: 0.469-30 ng/mL

  • Sample test of MP01044-1

    Sample test of MP01044-1

    The mean PROS1 concentration was determined to be 276.09 ng/mL in HuH-7 cell extract based on a 1.4 mg/mL extract load.

  • IHC staining of mouse brain using 84107-2-RR (same clone as 84107-2-PBS)

    IHC staining of mouse brain using 84107-2-RR (same clone as 84107-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84107-2-RR (PROS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84107-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 84107-2-RR (same clone as 84107-2-PBS)

    IHC staining of mouse brain using 84107-2-RR (same clone as 84107-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84107-2-RR (PROS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84107-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84107-2-RR (same clone as 84107-2-PBS)

    IF Staining of HepG2 using 84107-2-RR (same clone as 84107-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PROS1 antibody (84107-2-RR, Clone: 241241E5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84107-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84107-2-RR (same clone as 84107-2-PBS)

    IF Staining of HepG2 using 84107-2-RR (same clone as 84107-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PROS1 antibody (84107-2-RR, Clone: 241241E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84107-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01044-1

    Cytometric bead array standard curve of MP01044-1

    Cytometric bead array standard curve of MP01044-1, PROS1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84107-2-PBS. Detection antibody: 84107-1-PBS. Standard: Ag10539. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84107-2-RR

    Affinity and Kinetic Characterization of 84107-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84107-2-RR against Human PROS1 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

PROS1 Recombinant antibody, PBS Only (Capture)

PROS1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84107-2-PBS

产品说明书

CloneNo. 241241E5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

Vitamin K-dependent protein S, PROS, 241241E5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84107-2-PBS targets PROS1 as part of a matched antibody pair:

MP01044-1: 84107-2-PBS capture and 84107-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 PROS1 fusion protein Ag10539 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein S (alpha)
别名 Vitamin K-dependent protein S, PROS, 241241E5
计算分子量 75 kDa
观测分子量 75 kDa
GenBank蛋白编号BC015801
基因名称 PROS1
Gene ID (NCBI) 5627
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07225
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PROS1, also named as Vitamin K-dependent protein S, is a 676 amino acid protein, which contains 4 EGF-like domains, contains one Gla (gamma-carboxy-glutamate) domain and contains 2 laminin G-like domains. PROS1 as a secreted protein platelets alpha granules is expressed in plasma. PROS1 is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa and helps to prevent coagulation and stimulating fibrinolysis.