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TRAPPC4 Recombinant antibody, PBS Only (Detector)

TRAPPC4 Uni-rAbTM Recombinant Antibody for WB, IP, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241158C11

Cat no : 84119-3-PBS

Print datasheet

Synonyms

SBDN, PTD009, HSPC172, CGI-104, CGI 104

验证数据展示

  • WB analysis using 84119-3-RR (same clone as 84119-3-PBS)

    WB analysis using 84119-3-RR (same clone as 84119-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84119-3-RR (TRAPPC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84119-3-PBS in a different storage buffer formulation.

  • IP experiment of HL-60 using 84119-3-RR (same clone as 84119-3-PBS)

    IP experiment of HL-60 using 84119-3-RR (same clone as 84119-3-PBS)

    IP result of anti-TRAPPC4 (IP:84119-3-RR, 4ug; Detection:84119-3-RR 1:3000) with HL-60 cells lysate 2240 ug. This data was developed using the same antibody clone with 84119-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01030-2

    Cytometric bead array standard curve of MP01030-2

    Cytometric bead array standard curve of MP01030-2, TRAPPC4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84119-4-PBS. Detection antibody: 84119-3-PBS. Standard: Ag2737. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01030-3

    Cytometric bead array standard curve of MP01030-3

    Cytometric bead array standard curve of MP01030-3, TRAPPC4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84119-1-PBS. Detection antibody: 84119-3-PBS. Standard: Ag2737. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84119-3-RR

    Affinity and Kinetic Characterization of 84119-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84119-3-RR against Human TRAPPC4 were performed. The affinity constant is below 1 pM.

产品信息

84119-3-PBS targets TRAPPC4 as part of a matched antibody pair:

MP01030-2: 84119-4-PBS capture and 84119-3-PBS detection (validated in Cytometric bead array)

MP01030-3: 84119-1-PBS capture and 84119-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IP, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TRAPPC4 fusion protein Ag2737 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name trafficking protein particle complex 4
Synonyms SBDN, PTD009, HSPC172, CGI-104, CGI 104
Calculated Molecular Weight 219 aa, 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC010866
Gene Symbol TRAPPC4
Gene ID (NCBI) 51399
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y296
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transport protein particle (TRAPP, also known as trafficking protein particle), a multimeric guanine nucleotide-exchange factor, regulates multiple membrane trafficking pathways (PMID: 20966969). TRAPPC4, also known as synbindin, is a core component of the TRAPP complexes and one of the essential subunits for guanine nucleotide exchange factor activity for Rab1 GTPase (PMID: 31794024). Deficiencies in vesicular transport mediated by TRAPPC4 have been associated with severe syndromic intellectual disability (PMID: 31794024).