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验证数据展示

  • WB analysis using 84119-3-RR (same clone as 84119-3-PBS)

    WB analysis using 84119-3-RR (same clone as 84119-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84119-3-RR (TRAPPC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84119-3-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 84119-3-RR (same clone as 84119-3-PBS)

    WB analysis of MCF-7 using 84119-3-RR (same clone as 84119-3-PBS)

    WB result of TRAPPC4 antibody (84119-3-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAPPC4 transfected MCF-7 cells. This data was developed using the same antibody clone with 84119-3-PBS in a different storage buffer formulation.

  • IP experiment of HL-60 using 84119-3-RR (same clone as 84119-3-PBS)

    IP experiment of HL-60 using 84119-3-RR (same clone as 84119-3-PBS)

    IP result of anti-TRAPPC4 (IP:84119-3-RR, 4ug; Detection:84119-3-RR 1:3000) with HL-60 cells lysate 2240 ug. This data was developed using the same antibody clone with 84119-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01030-2

    Cytometric bead array standard curve of MP01030-2

    Cytometric bead array standard curve of MP01030-2, TRAPPC4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84119-4-PBS. Detection antibody: 84119-3-PBS. Standard: Ag2737. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01030-3

    Cytometric bead array standard curve of MP01030-3

    Cytometric bead array standard curve of MP01030-3, TRAPPC4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84119-1-PBS. Detection antibody: 84119-3-PBS. Standard: Ag2737. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84119-3-RR

    Affinity and Kinetic Characterization of 84119-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84119-3-RR against Human TRAPPC4 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

TRAPPC4 Recombinant antibody, PBS Only (Detector)

TRAPPC4 Uni-rAbTM Recombinant Antibody for WB, IP, Cytometric bead array, Indirect ELISA
Cat No. 84119-3-PBS

产品说明书

CloneNo. 241158C11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, Cytometric bead array, Indirect ELISA

别名

SBDN, PTD009, HSPC172, CGI-104, CGI 104

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84119-3-PBS targets TRAPPC4 as part of a matched antibody pair:

MP01030-2: 84119-4-PBS capture and 84119-3-PBS detection (validated in Cytometric bead array)

MP01030-3: 84119-1-PBS capture and 84119-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IP, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TRAPPC4 fusion protein Ag2737 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 trafficking protein particle complex 4
别名 SBDN, PTD009, HSPC172, CGI-104, CGI 104
计算分子量 219 aa, 24 kDa
观测分子量 24 kDa
GenBank蛋白编号BC010866
基因名称 TRAPPC4
Gene ID (NCBI) 51399
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y296
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transport protein particle (TRAPP, also known as trafficking protein particle), a multimeric guanine nucleotide-exchange factor, regulates multiple membrane trafficking pathways (PMID: 20966969). TRAPPC4, also known as synbindin, is a core component of the TRAPP complexes and one of the essential subunits for guanine nucleotide exchange factor activity for Rab1 GTPase (PMID: 31794024). Deficiencies in vesicular transport mediated by TRAPPC4 have been associated with severe syndromic intellectual disability (PMID: 31794024).