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CD117/c-Kit Recombinant antibody, PBS Only (Capture)

CD117/c-Kit Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241312A12

Cat no : 84259-3-PBS

Print datasheet

Synonyms

CD117, KIT, EC:2.7.10.1, c-Kit, C Kit

验证数据展示

  • IHC staining of human colon using 84259-3-RR (same clone as 84259-3-PBS)

    IHC staining of human colon using 84259-3-RR (same clone as 84259-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84259-3-RR (CD117/c-Kit antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84259-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD117/c-kit antibody included
  • IF Staining of Mo7e using 84259-3-RR (same clone as 84259-3-PBS)

    IF Staining of Mo7e using 84259-3-RR (same clone as 84259-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Mo7e cells using CD117/c-Kit antibody (84259-3-RR, Clone: 241312A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84259-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01171-1

    Cytometric bead array standard curve of MP01171-1

    Cytometric bead array standard curve of MP01171-1, CD117/c-Kit Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84259-3-PBS. Detection antibody: 84259-2-PBS. Standard: RP02323. Range: 0.156-20 ng/mL.

  • Cytometric bead array standard curve of MP01171-2

    Cytometric bead array standard curve of MP01171-2

    Cytometric bead array standard curve of MP01171-2, CD117/c-Kit Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84259-3-PBS. Detection antibody: 84259-1-PBS. Standard: RP02323. Range: 0.156-20 ng/mL.

  • Affinity and Kinetic Characterization of 84259-3-RR

    Affinity and Kinetic Characterization of 84259-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84259-3-RR against Human CD117/c-Kit were performed. The affinity constant is 0.519 nM.

Planning an IHC experiment? We recommend our IHCeasy CD117/c-kit Ready-To-Use IHC Kit. CD117/c-kit primary antibody included.

产品信息

84259-3-PBS targets CD117/c-Kit as part of a matched antibody pair:

MP01171-1: 84259-3-PBS capture and 84259-2-PBS detection (validated in Cytometric bead array)

MP01171-2: 84259-3-PBS capture and 84259-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Synonyms CD117, KIT, EC:2.7.10.1, c-Kit, C Kit
Calculated Molecular Weight 976 aa, 110 kDa
GenBank Accession NumberBC071593
Gene Symbol CD117
Gene ID (NCBI) 3815
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10721
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early hemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).